Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | highest activity is measured using the following buffer composition: sodium phosphate + Triton X-100 (1%) + PVP (0.5%) + EDTA (1.0 mM) + sodium thiosulfate (0.25%) | Gossypium hirsutum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | markedly inhibits PPO | Gossypium hirsutum | |
Ba2+ | moderately inhibits PPO | Gossypium hirsutum | |
Cu2+ | moderately inhibits PPO | Gossypium hirsutum | |
dithiothreitol | markedly inhibits PPO | Gossypium hirsutum | |
FeCl3 | markedly inhibits PPO | Gossypium hirsutum | |
SnCl2 | markedly inhibits PPO | Gossypium hirsutum | |
Sodium metabisulfite | markedly inhibits PPO | Gossypium hirsutum | |
Zn2+ | moderately inhibits PPO | Gossypium hirsutum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | highly activates PPO | Gossypium hirsutum | |
Mg2+ | highly activates PPO | Gossypium hirsutum | |
Mn2+ | highly activates PPO | Gossypium hirsutum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Gossypium hirsutum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gossypium hirsutum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a Sephadex G-200 column balanced with 0.1 M sodium phosphate buffer (pH 6.5) | Gossypium hirsutum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | coker 312 seeds are used. Cotton cell suspensions are obtained from calli derived from hypocotyl segments of plant. Coker 312 cell suspensions show the development of embryogenic structures | Gossypium hirsutum | - |
cell suspension culture | R405-2000 seeds are used. Cotton cell suspensions are obtained from calli derived from hypocotyl segments of plant. R405-2000 cell suspensions does not show the development of embryogenic structures | Gossypium hirsutum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
compared to PPO from R405-2000, a nonembryogenic cultivar, PPO from coker 312 (embryogenic cultivar) shows a higher PPO activity increasing markedly form primary culture to third subculture | Gossypium hirsutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 catechol + O2 | enzyme shows high affinity to this substrate | Gossypium hirsutum | 2 1,2-benzoquinone + 2 H2O | - |
? | |
2 catechol + O2 | enzyme shows highest affinity to this substrate | Gossypium hirsutum | 2 1,2-benzoquinone + 2 H2O | - |
? | |
4-methylcatechol + O2 | enzyme shows high affinity to this substrate | Gossypium hirsutum | ? | - |
? | |
4-methylcatechol + O2 | enzyme shows highest affinity to this substrate | Gossypium hirsutum | ? | - |
? | |
catechin + O2 | enzyme shows moderate affinity to this substrate | Gossypium hirsutum | ? | - |
? | |
dopamine + O2 | enzyme shows moderate affinity to this substrate | Gossypium hirsutum | ? | - |
? | |
gallic acid + O2 | enzyme shows very low affinity to this substrate | Gossypium hirsutum | ? | - |
? | |
additional information | PPO shows activity with biphenols, triphenol but not with monophenols | Gossypium hirsutum | ? | - |
? | |
pyrogallol + O2 | enzyme shows low affinity to this substrate | Gossypium hirsutum | ? | - |
? | |
tetramethylbenzidine + O2 | enzyme shows low affinity to this substrate | Gossypium hirsutum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Gossypium hirsutum |
PPO | from Coker 312, an embryogenic cultivar | Gossypium hirsutum |
PPO | from R405-2000, an nonembryogenic cultivar | Gossypium hirsutum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Gossypium hirsutum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | 60 min | Gossypium hirsutum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
optimal pH, highest activity is measured using the following buffer composition: sodium phosphate + Triton X-100 (1%) + PVP (0.5%) + EDTA (1.0 mM) + sodium thiosulfate (0.25%) | Gossypium hirsutum |
6.5 | - |
assay at | Gossypium hirsutum |
7 | - |
optimal pH, highest activity is measured using the following buffer composition: sodium phosphate + Triton X-100 (1%) + PVP (0.5%) + EDTA (1.0 mM) + sodium thiosulfate (0.25%) | Gossypium hirsutum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | enzyme retains 95% of its activity | Gossypium hirsutum |
6 | 7 | enzyme retains 95% of its activity | Gossypium hirsutum |