Literature summary for 1.10.3.1 extracted from

Perdomo-Morales, R.; Montero-Alejo, V.; Perera, E.; Pardo-Ruiz, Z.; Alonso-Jimenez, E.
Hemocyanin-derived phenoloxidase activity in the spiny lobster Panulirus argus (Latreille, 1804) (2008), Biochim. Biophys. Acta, 1780, 652-658.

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Inhibitors

Inhibitors	Comment	Organism	Structure
catechol	catechol produces substrate inhibition above 16 mM	Panulirus argus
dopamine	dopamine produces substrate inhibition above 2 mM	Panulirus argus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.181	-	dopamine	SDS-activated hemocyanin	Panulirus argus
2.565	-	L-Dopa	SDS-activated hemocyanin	Panulirus argus
7.174	-	catechol	SDS-activated hemocyanin	Panulirus argus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
77000	-	1 * 77000 + 1 * 80000 + 1 * 89000	Panulirus argus
80000	-	1 * 77000 + 1 * 80000 + 1 * 89000	Panulirus argus
89000	-	1 * 77000 + 1 * 80000 + 1 * 89000	Panulirus argus
170000	-	incubation of native hemocyanin with trypsin, produces a band of 170000 Da with intense phenoloxidase activity	Panulirus argus
190000	-	incubation of native hemocyanin with chymotrypsin, produces a band of 190000 Da with intense phenoloxidase activity	Panulirus argus

Organism

Organism	UniProt	Comment	Textmining
Panulirus argus	-	spiny lobster	-

Purification (Commentary)

Purification (Comment)	Organism
Sephacryl S-300 gel filtration	Panulirus argus

Source Tissue

Source Tissue	Comment	Organism	Textmining
hemolymph	-	Panulirus argus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 catechol + O2	-	Panulirus argus	2 1,2-benzoquinone + 2 H2O	-	?
2 dopamine + O2	dopamine is the most specific substrate	Panulirus argus	2 4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O	-	?
L-Dopa + O2	-	Panulirus argus	L-dopaquinone + H2O	-	?
additional information	phenoloxidase activity is not observed when tyramine and tyrosine (monophenols) were used as substrates	Panulirus argus	?	-	?

Subunits

Subunits	Comment	Organism
trimer	1 * 77000 + 1 * 80000 + 1 * 89000	Panulirus argus

Synonyms

Synonyms	Comment	Organism
hemocyanin	behaves as a catecholoxidase and shows phenoloxidase activity in vitro	Panulirus argus
phenoloxidase	-	Panulirus argus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	there are two peaks of optimal pH, at pH 5.5 and pH 7.0	Panulirus argus
7	-	there are two peaks of optimal pH, at pH 5.5 and pH 7.0	Panulirus argus

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Release 2023.1 (January 2023)