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Literature summary for 1.10.3.1 extracted from

  • Jaenicke, E.; Decker, H.
    Kinetic properties of catecholoxidase activity of tarantula hemocyanin (2008), FEBS J., 275, 1518-1528.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
SDS after the addition of SDS, the catecholoxidase activity of Eurypelma hemocyanin develops gradually over a period of 5 min until a constant activity is reached Aphonopelma californicum (nom. dub.)

General Stability

General Stability Organism
The activated state of Eurypelma hemocyanin is stable for several hours. Aphonopelma californicum (nom. dub.)

Inhibitors

Inhibitors Comment Organism Structure
azide typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin Aphonopelma californicum (nom. dub.)
dopamine at high dopamine concentration, a decrease in activity is observed, indicating substrate inhibition Aphonopelma californicum (nom. dub.)
kojic acid typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin Aphonopelma californicum (nom. dub.)
L-mimosine typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin Aphonopelma californicum (nom. dub.)
Phenylthiourea typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin Aphonopelma californicum (nom. dub.)
tyramine typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin Aphonopelma californicum (nom. dub.)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.22
-
dopamine at substrate concentrations above 4 mM, substrate inhibition is observed and the data are fitted to a simple model for substrate inhibition. KM, kcat and KI are obtained for the inhibitory binding site Aphonopelma californicum (nom. dub.)
1.45
-
dopamine substrate range 0.3-4 mM Aphonopelma californicum (nom. dub.)

Metals/Ions

Metals/Ions Comment Organism Structure
copper
-
Aphonopelma californicum (nom. dub.)

Organism

Organism UniProt Comment Textmining
Aphonopelma californicum (nom. dub.)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aphonopelma californicum (nom. dub.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 catechol + O2
-
Aphonopelma californicum (nom. dub.) 2 1,2-benzoquinone + 2 H2O
-
?
dopamine + O2
-
Aphonopelma californicum (nom. dub.) dopaminequinone + H2O
-
?
epinephrine + O2
-
Aphonopelma californicum (nom. dub.) ?
-
?
L-dihydroxyphenylalanine + O2 L-dopa Aphonopelma californicum (nom. dub.) L-dopaquinone + H2O
-
?
additional information to investigate the substrate specificity of activated hemocyanin, the turnover of several diphenols is compared using an oxygen electrode Aphonopelma californicum (nom. dub.) ?
-
?
N-acetyldopamine + O2 NADA Aphonopelma californicum (nom. dub.) ?
-
?
norepinephrine + O2
-
Aphonopelma californicum (nom. dub.) ?
-
?

Synonyms

Synonyms Comment Organism
catecholoxidase
-
Aphonopelma californicum (nom. dub.)
hemocyanin
-
Aphonopelma californicum (nom. dub.)
phenoloxidase
-
Aphonopelma californicum (nom. dub.)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.7
-
dopamine at substrate concentrations above 4 mM, substrate inhibition is observed and the data are fitted to a simple model for substrate inhibition. KM, kcat and KI are obtained for the inhibitory binding site Aphonopelma californicum (nom. dub.)
6
-
dopamine substrate range 0.3-4 mM Aphonopelma californicum (nom. dub.)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Aphonopelma californicum (nom. dub.)

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information fitting attempts reveal that inhibition by phenylthiourea and azide is not in accordance with a competitive binding model, the binding affinity of the inhibitors can not be determined by fitting the data Aphonopelma californicum (nom. dub.)
0.0119
-
kojic acid with dopamine as a substrate Aphonopelma californicum (nom. dub.)
0.0477
-
L-mimosine with dopamine as a substrate Aphonopelma californicum (nom. dub.)
0.2782
-
tyramine with dopamine as a substrate Aphonopelma californicum (nom. dub.)
29.64
-
dopamine at substrate concentrations above 4 mM, substrate inhibition is observed and the data are fitted to a simple model for substrate inhibition. KM, kcat and KI are obtained for the inhibitory binding site Aphonopelma californicum (nom. dub.)