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Literature summary for 1.10.3.1 extracted from

  • Wuyts, N.; De Waele, D.; Swennen, R.
    Extraction and partial characterization of polyphenol oxidase from banana (Musa acuminata Grande naine) roots (2006), Plant Physiol. Biochem., 44, 308-314.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxybenzoic acid
-
Musa acuminata
Citric acid slight inhibition Musa acuminata
dithiothreitol complete inhibition at 0.150 mM of the root enzyme Musa acuminata
EDTA inhibits the root enzyme, while the pulp enzyme is only poorly inhiibited Musa acuminata
L-ascorbic acid complete inhibition at 0.90 mM of the root enzyme, and at 1 mM of the pulp enzyme Musa acuminata
L-cysteine complete inhibition at 0.973 mM of the root enzyme, and at 1 mM of the pulp enzyme Musa acuminata
Sodium metabisulfite complete inhibition at 0.109 mM of the root enzyme, and at 1 mM of the pulp enzyme Musa acuminata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Musa acuminata
0.6
-
dopamine pH 6.5, 25°C Musa acuminata
1.2
-
D-catechin pH 6.5, 25°C Musa acuminata
6.2
-
chlorogenic acid pH 6.5, 25°C Musa acuminata
8.3
-
4-methylcatechol pH 6.5, 25°C Musa acuminata
9.5
-
caffeic acid pH 6.5, 25°C Musa acuminata
56.3
-
L-Dopa pH 6.5, 25°C Musa acuminata
69
-
catechol pH 6.5, 25°C Musa acuminata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-catechin + O2 Musa acuminata
-
?
-
?
dopamine + O2 Musa acuminata
-
?
-
?
additional information Musa acuminata the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plant’s defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis ?
-
?

Organism

Organism UniProt Comment Textmining
Musa acuminata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
optimization of enzyme extraction from roots, best using 0.2 M phosphate buffer at pH 7.0 with 5% insoluble polyvinylpyrrolidone and 0.25% Triton X-100, overview Musa acuminata

Source Tissue

Source Tissue Comment Organism Textmining
fruit pulp and peel Musa acuminata
-
leaf
-
Musa acuminata
-
additional information tissue distribution, overview Musa acuminata
-
root aerenchyma in the cortex Musa acuminata
-
stem
-
Musa acuminata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Musa acuminata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcatechol + O2
-
Musa acuminata 4-methyl-1,2-benzoquinone + H2O
-
?
caffeic acid + O2
-
Musa acuminata ?
-
?
catechol + 1/2 O2 low activity Musa acuminata 1,2-benzoquinone + H2O
-
?
chlorogenic acid + O2
-
Musa acuminata ?
-
?
D-catechin + O2
-
Musa acuminata ?
-
?
D-catechin + O2 best substrate Musa acuminata ?
-
?
dopamine + O2
-
Musa acuminata ?
-
?
L-dopa + 1/2 O2 i.e. L-3,4-dihydroxyphenylalanine, low activity Musa acuminata L-dopaquinone + H2O
-
?
additional information the enzyme plays a role in enzymatic browning, rapid discolouration of leaf, stem and root tissue after injury and strong pigmentation of tissue extracts, PPO and phenolic compounds could be an important part of the plant’s defence system against pests and diseases, including root parasitic nematodes, e.g. Radopholus similis Musa acuminata ?
-
?
additional information the enzyme catalyzes the hydroxylation of monophenols to o-diphenols, monophenolase activity EC 1.14.18.1, and the oxidation of the o-diphenols to o-quinones, diphenolase activity EC 1.10.3.1, cross-reaction analysis, overview Musa acuminata ?
-
?

Synonyms

Synonyms Comment Organism
catecholase
-
Musa acuminata
More cf. 1.14.18.1 Musa acuminata
o-diphenoloxidase
-
Musa acuminata
polyphenol oxidase
-
Musa acuminata
PPO
-
Musa acuminata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Musa acuminata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Musa acuminata