Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chymotrypsin | proteolytic 2.5fold activation | Scyllarides latus | |
Trypsin | proteolytic 3.5fold activation | Scyllarides latus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
kojic acid | 99% inhibition of diphenolase activity at 1 mM | Scyllarides latus | |
papain | proteolytic inactivation | Scyllarides latus | |
tropolone | 97% inhibition of diphenolase activity at 1 mM | Scyllarides latus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.427 | - |
dopamine | pH 6.5, 25°C | Scyllarides latus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Scyllarides latus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
6 * 78000, SDS-PAGE | Scyllarides latus |
440000 | - |
gel filtration | Scyllarides latus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scyllarides latus | - |
mediterranean slipper lobster | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | proteolytic activation by trypsin and chymotrypsin, proteolytic inactivation by papain | Scyllarides latus |
Purification (Comment) | Organism |
---|---|
native tyrosinase from hemolymph to homogeneity by anion exchange and hydrophobic interaction chromatography | Scyllarides latus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hemolymph | - |
Scyllarides latus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dopamine + O2 | - |
Scyllarides latus | ? | - |
? | |
additional information | the purified tyrosinase from hemolymph shows both monophenolase, EC 1.14.18.1, and diphenolase, EC 1.10.3.1, activity and therefore it can be defined as a true tyrosinase, the purified hemocynin does not show any tyrosinase activity | Scyllarides latus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 78000, SDS-PAGE | Scyllarides latus |
Synonyms | Comment | Organism |
---|---|---|
catecholase | - |
Scyllarides latus |
CO | - |
Scyllarides latus |
More | cf. EC 1.14.18.1 | Scyllarides latus |
o-diphenol: dioxygen oxidoreductase, dehydrogenating | - |
Scyllarides latus |
phenoloxidase | - |
Scyllarides latus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Scyllarides latus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
282 | - |
dopamine | pH 6.5, 25°C | Scyllarides latus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Scyllarides latus |