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Literature summary for 1.10.3.1 extracted from
- Purification and characterization of Ocimum basilicum L. polyphenol oxidase (2005), J. Agric. Food Chem., 53, 10224-10230.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Ocimum basilicum | |
| 1.62 | - |
catechol | pH 8.0, 25°C | Ocimum basilicum |  |
| 2.77 | - |
4-methylcatechol | pH 6.0, 25°C | Ocimum basilicum | |
| 3.42 | - |
pyrogallol | pH 9.0, 25°C | Ocimum basilicum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ocimum basilicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 11.5fold by (NH4)2SO4 precipitation, dialysis, and L-tyrosine-4-aminobenzoic acid affinity chromatography | Ocimum basilicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 59.46 | - |
purified enzyme | Ocimum basilicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylcatechol + O2 | - |
Ocimum basilicum | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
| catechol + 1/2 O2 | - |
Ocimum basilicum | 1,2-benzoquinone + H2O | - |
? | |
| pyrogallol + O2 | - |
Ocimum basilicum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| diphenolase | - |
Ocimum basilicum |
| polyphenol oxidase | - |
Ocimum basilicum |
| PPO | - |
Ocimum basilicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
with substrate 4-methylcatechol | Ocimum basilicum |
| 40 | - |
with substrate catechol | Ocimum basilicum |
| 50 | - |
with substrate pyrogallol | Ocimum basilicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 60 | - |
Ocimum basilicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
heat denaturation kinetics | Ocimum basilicum |
| 55 | - |
30 min, purified enzyme, loss of 50% activity | Ocimum basilicum |
| 75 | - |
30 min, purified enzyme, loss of 55%, 80%, and 70% activity with substrates 4-methylcatechol, catechol, and pyrogallol, respectively | Ocimum basilicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
with substrate 4-methylcatechol | Ocimum basilicum |
| 8 | - |
with substrate catechol | Ocimum basilicum |
| 9 | - |
with substrate pyrogallol | Ocimum basilicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | pH-profile | Ocimum basilicum |
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Release 2023.1 (January 2023)
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