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Literature summary for 1.10.3.1 extracted from
- The purification of polyphenol oxidase from tobacco (2002), Protein Expr. Purif., 24, 51-55.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
catechol | - |
Nicotiana tabacum |  |
| 1.2 | - |
catechol | pH 6.5 | Nicotiana tabacum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36500 | - |
1 * 36500, MALDI-TOF mass spectrometry | Nicotiana tabacum |
| 42000 | - |
1 * 42000, SDS-PAGE | Nicotiana tabacum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Nicotiana tabacum | PPO II inhibits cultures of Escherichia coli and it accumulates on the wounded sites of tobacco leaves indicating that it may act as a defense role in plat defense system | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | - |
- |
- |
| Nicotiana tabacum | - |
polyphenol oxidase II, enzyme might act as an important defense system against the spread of pathogens at wounded sites | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Nicotiana tabacum |
| ammonium sulfate, DEAE-Sephadex A-50, Sephadex G-75 | Nicotiana tabacum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Nicotiana tabacum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Nicotiana tabacum |
| additional information | - |
4500 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.01 per min | Nicotiana tabacum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| catechol + 1/2 O2 | - |
Nicotiana tabacum | 1,2-benzoquinone + H2O | - |
? | |
| catechol + 1/2 O2 | no activity with tyrosine, o-methoxyphenol, p-catechol and m-catechol | Nicotiana tabacum | 1,2-benzoquinone + H2O | - |
? | |
| chlorogenic acid + O2 | - |
Nicotiana tabacum | ? | - |
? | |
| chlorogenic acid + O2 | 50% of activity with catechol | Nicotiana tabacum | ? | - |
? | |
| additional information | PPO II inhibits cultures of Escherichia coli and it accumulates on the wounded sites of tobacco leaves indicating that it may act as a defense role in plat defense system | Nicotiana tabacum | ? | - |
? | |
| additional information | no activity with tyrosine, o-methoxyphenol, p-diphenol and m-diphenol | Nicotiana tabacum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Nicotiana tabacum |
| monomer | 1 * 36500, MALDI-TOF mass spectrometry | Nicotiana tabacum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPO II | - |
Nicotiana tabacum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Nicotiana tabacum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 65 | 94% of maximal activity at 30°C, 78% of maximal activity at 20°C, rapid decline of activity above 65°C | Nicotiana tabacum |
| 20 | 65 | enzyme retains more than 50% of its activity from 20°C to 65°C | Nicotiana tabacum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Nicotiana tabacum |
| 6 | - |
rapid decrease in activity at higher and lower pH | Nicotiana tabacum |
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Release 2023.1 (January 2023)
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