KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
catechol | - |
Nicotiana tabacum | |
1.2 | - |
catechol | pH 6.5 | Nicotiana tabacum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
1 * 36500, MALDI-TOF mass spectrometry | Nicotiana tabacum |
42000 | - |
1 * 42000, SDS-PAGE | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nicotiana tabacum | PPO II inhibits cultures of Escherichia coli and it accumulates on the wounded sites of tobacco leaves indicating that it may act as a defense role in plat defense system | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Nicotiana tabacum | - |
polyphenol oxidase II, enzyme might act as an important defense system against the spread of pathogens at wounded sites | - |
Purification (Comment) | Organism |
---|---|
- |
Nicotiana tabacum |
ammonium sulfate, DEAE-Sephadex A-50, Sephadex G-75 | Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Nicotiana tabacum |
additional information | - |
4500 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.01 per min | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + 1/2 O2 | - |
Nicotiana tabacum | 1,2-benzoquinone + H2O | - |
? | |
catechol + 1/2 O2 | no activity with tyrosine, o-methoxyphenol, p-catechol and m-catechol | Nicotiana tabacum | 1,2-benzoquinone + H2O | - |
? | |
chlorogenic acid + O2 | - |
Nicotiana tabacum | ? | - |
? | |
chlorogenic acid + O2 | 50% of activity with catechol | Nicotiana tabacum | ? | - |
? | |
additional information | PPO II inhibits cultures of Escherichia coli and it accumulates on the wounded sites of tobacco leaves indicating that it may act as a defense role in plat defense system | Nicotiana tabacum | ? | - |
? | |
additional information | no activity with tyrosine, o-methoxyphenol, p-diphenol and m-diphenol | Nicotiana tabacum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Nicotiana tabacum |
monomer | 1 * 36500, MALDI-TOF mass spectrometry | Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
PPO II | - |
Nicotiana tabacum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Nicotiana tabacum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 65 | 94% of maximal activity at 30°C, 78% of maximal activity at 20°C, rapid decline of activity above 65°C | Nicotiana tabacum |
20 | 65 | enzyme retains more than 50% of its activity from 20°C to 65°C | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Nicotiana tabacum |
6 | - |
rapid decrease in activity at higher and lower pH | Nicotiana tabacum |