Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-hydroxy-2,4,6-cycloheptatrien-1-one | trivial name tropolone | Lablab purpureus | |
ascorbic acid | - |
Lablab purpureus | |
cysteine | - |
Lablab purpureus | |
Metabisulfite | - |
Lablab purpureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.18 | - |
L-Dopa | - |
Lablab purpureus | |
4 | - |
4-methylcatechol | - |
Lablab purpureus | |
10.5 | - |
catechol | - |
Lablab purpureus | |
12.5 | - |
pyrogallol | - |
Lablab purpureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
4 * 30000, SDS-PAGE | Lablab purpureus |
120000 | - |
gel filtration | Lablab purpureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lablab purpureus | - |
field bean | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Sephacel, Phenyl-agarose, Sephadex G-100 | Lablab purpureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
68077.0 units/mg, 1 unit is defined as absorbance change of 0.001 per min at 25°C | Lablab purpureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylcatechol + O2 | 140% of activity with catechol | Lablab purpureus | 4-methyl-1,2-benzoquinone | - |
? | |
catechol + 1/2 O2 | - |
Lablab purpureus | 1,2-benzoquinone + H2O | - |
? | |
L-dopa + 1/2 O2 | 22.65 of activity with catechol | Lablab purpureus | L-dopaquinone + H2O | - |
? | |
pyrogallol + O2 | 24% of activity with catechol | Lablab purpureus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 30000, SDS-PAGE | Lablab purpureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
catechol oxidation | Lablab purpureus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5.5 | 50% activity at pH 3.5 and pH 5.5, 25% activity at pH 3.0 | Lablab purpureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00058 | - |
tropolone | - |
Lablab purpureus | |
0.0055 | - |
Metabisulfite | - |
Lablab purpureus | |
0.0075 | - |
ascorbic acid | - |
Lablab purpureus | |
19.5 | - |
cysteine | - |
Lablab purpureus |