Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris KM71H | Sarocladium strictum |
Protein Variants | Comment | Organism |
---|---|---|
Y300A | the variant shows broader substrate range and higher H2O2 stability and specifically exhibits up to 40times higher activity on all tested sugars except D-glucose compared to the wild type enzyme | Sarocladium strictum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | about 40% inhibition at 200 mM | Sarocladium strictum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
cellobiose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
0.25 | - |
cellotriose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
3.15 | - |
xylotriose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
5.11 | - |
xylobiose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
8.1 | - |
D-glucose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
51.8 | - |
D-xylose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sarocladium strictum | D7PF15 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Sarocladium strictum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabinoxylan + O2 | - |
Sarocladium strictum | ? | - |
? | |
beech wood xylan + O2 | - |
Sarocladium strictum | ? | - |
? | |
cellobiose + O2 | - |
Sarocladium strictum | cellobiono-1,5-lactone + H2O2 | - |
? | |
cellotriose + O2 | - |
Sarocladium strictum | cellotriono-1,5-lactone + H2O2 | - |
? | |
D-glucose + O2 | - |
Sarocladium strictum | D-glucono-1,5-lactone + H2O2 | - |
? | |
D-xylose + O2 | - |
Sarocladium strictum | D-xylono-1,5-lactone + H2O2 | - |
? | |
maltose + O2 | - |
Sarocladium strictum | maltono-1,5-lactone + H2O2 | - |
? | |
propoxylated wheat bran hemicellulose + O2 | - |
Sarocladium strictum | ? | - |
? | |
wheat bran hemicellulose + O2 | - |
Sarocladium strictum | ? | - |
? | |
xylobiose + O2 | highest activity | Sarocladium strictum | xylobiono-1,5-lactone + H2O2 | - |
? | |
xylotriose + O2 | - |
Sarocladium strictum | xylotriono-1,5-lactone + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gluco-oligosaccharide oxidase | - |
Sarocladium strictum |
GOOX | - |
Sarocladium strictum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.2 | - |
cellotriose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
11.3 | - |
D-xylose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
13.2 | - |
D-glucose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
13.3 | - |
xylobiose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
13.7 | - |
cellobiose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
13.9 | - |
xylotriose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
D-xylose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
1.6 | - |
D-glucose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
2.6 | - |
xylobiose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
4.3 | - |
xylotriose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
45 | - |
cellotriose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum | |
56.7 | - |
cellobiose | mutant enzyme Y300A, at pH 8.0 and 37°C | Sarocladium strictum |