Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.99.B3 extracted from

  • Vuong, T.V.; Foumani, M.; MacCormick, B.; Kwan, R.; Master, E.R.
    Direct comparison of gluco-oligosaccharide oxidase variants and glucose oxidase: substrate range and H2O2 stability (2016), Sci. Rep., 6, 37356.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris KM71H Sarocladium strictum

Protein Variants

Protein Variants Comment Organism
Y300A the variant shows broader substrate range and higher H2O2 stability and specifically exhibits up to 40times higher activity on all tested sugars except D-glucose compared to the wild type enzyme Sarocladium strictum

Inhibitors

Inhibitors Comment Organism Structure
H2O2 about 40% inhibition at 200 mM Sarocladium strictum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
cellobiose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
0.25
-
cellotriose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
3.15
-
xylotriose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
5.11
-
xylobiose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
8.1
-
D-glucose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
51.8
-
D-xylose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum

Organism

Organism UniProt Comment Textmining
Sarocladium strictum D7PF15
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Sarocladium strictum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinoxylan + O2
-
Sarocladium strictum ?
-
?
beech wood xylan + O2
-
Sarocladium strictum ?
-
?
cellobiose + O2
-
Sarocladium strictum cellobiono-1,5-lactone + H2O2
-
?
cellotriose + O2
-
Sarocladium strictum cellotriono-1,5-lactone + H2O2
-
?
D-glucose + O2
-
Sarocladium strictum D-glucono-1,5-lactone + H2O2
-
?
D-xylose + O2
-
Sarocladium strictum D-xylono-1,5-lactone + H2O2
-
?
maltose + O2
-
Sarocladium strictum maltono-1,5-lactone + H2O2
-
?
propoxylated wheat bran hemicellulose + O2
-
Sarocladium strictum ?
-
?
wheat bran hemicellulose + O2
-
Sarocladium strictum ?
-
?
xylobiose + O2 highest activity Sarocladium strictum xylobiono-1,5-lactone + H2O2
-
?
xylotriose + O2
-
Sarocladium strictum xylotriono-1,5-lactone + H2O2
-
?

Synonyms

Synonyms Comment Organism
gluco-oligosaccharide oxidase
-
Sarocladium strictum
GOOX
-
Sarocladium strictum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.2
-
cellotriose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
11.3
-
D-xylose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
13.2
-
D-glucose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
13.3
-
xylobiose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
13.7
-
cellobiose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
13.9
-
xylotriose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.22
-
D-xylose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
1.6
-
D-glucose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
2.6
-
xylobiose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
4.3
-
xylotriose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
45
-
cellotriose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum
56.7
-
cellobiose mutant enzyme Y300A, at pH 8.0 and 37°C Sarocladium strictum