General Stability | Organism |
---|---|
Triton X-100 stabilizes | Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | metal chelators; not: sulfhydryl reagents | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
2-dehydro-D-gluconate | reaction with ferricyanide | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Gluconobacter oxydans | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE | Gluconobacter oxydans |
47000 | - |
1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE | Gluconobacter oxydans |
61000 | - |
1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.247 | - |
- |
Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-D-gluconate + ferricyanide | - |
Gluconobacter oxydans | 2,5-didehydro-D-gluconate + ferrocyanide | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE | Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
39 | - |
- |
Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Gluconobacter oxydans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | stable from pH 5.0 to pH 8.0 | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome | contains 0.91 mol of tightly bound c-type cytochrome per mol of enzyme | Gluconobacter oxydans | |
flavin | flavoprotein | Gluconobacter oxydans | |
flavin | contains 0.44 mol of covalently bound flavin per mol of enzyme | Gluconobacter oxydans |