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Literature summary for 1.1.99.37 extracted from

  • Park, H.; Lee, H.; Ro, Y.T.; Kim, Y.M.
    Identification and functional characterization of a gene for the methanol: N,N'-dimethyl-4-nitrosoaniline oxidoreductase from Mycobacterium sp. strain JC1 (DSM 3803) (2010), Microbiology, 156, 463-471.
    View publication on PubMed
Search for more literature for 1.1.99.37

Cloned(Commentary)

Cloned (Comment) Organism
-
 Mycobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 x * 46000, SDS-PAGE, x * 46223, calculated Mycobacterium sp.
46223
-
 x * 46000, SDS-PAGE, x * 46223, calculated Mycobacterium sp.

Organism

Organism UniProt Comment Textmining
Mycobacterium sp. C5MRT8
-
-
Mycobacterium sp. DSM 3803 / JC1 C5MRT8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.35
-
 substrate formaldehyde Mycobacterium sp.
0.37
-
 substrate ethanol Mycobacterium sp.
0.48
-
 substrate N,N-dimethyl-4-nitrosoaniline Mycobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + N,N-dimethyl-4-nitrosoaniline
-
 Mycobacterium sp. acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 r
ethanol + N,N-dimethyl-4-nitrosoaniline
-
 Mycobacterium sp. DSM 3803 / JC1 acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 r
ethanol + N,N-dimethyl-4-nitrosoaniline
-
 Mycobacterium sp. DSM 3803 acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 r
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 Mycobacterium sp. methanol + N,N-dimethyl-4-nitrosoaniline
-
 r
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 Mycobacterium sp. DSM 3803 / JC1 methanol + N,N-dimethyl-4-nitrosoaniline
-
 r
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 Mycobacterium sp. DSM 3803 methanol + N,N-dimethyl-4-nitrosoaniline
-
 r
methanol + N,N-dimethyl-4-nitrosoaniline
-
 Mycobacterium sp. formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 r
methanol + N,N-dimethyl-4-nitrosoaniline
-
 Mycobacterium sp. DSM 3803 / JC1 formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 r
methanol + N,N-dimethyl-4-nitrosoaniline
-
 Mycobacterium sp. DSM 3803 formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
-
 r

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE, x * 46223, calculated Mycobacterium sp.

Synonyms

Synonyms Comment Organism
MDO
-
 Mycobacterium sp.
methanol:N,N-dimethyl-4-nitrosoaniline oxidoreductase
-
 Mycobacterium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Mycobacterium sp. calculated
-
 5.4

Expression

Organism Comment Expression
Mycobacterium sp. transcription of methanol:N,N-dimethyl-4-nitrosoaniline oxidoreductase is upregulated in cells growing on methanol compared with cells growing on glucose up

General Information

General Information Comment Organism
physiological function an enzyme-deficient mutant strain does not grow with methanol as a sole source of carbon and energy Mycobacterium sp.