Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus methanolicus |
Protein Variants | Comment | Organism |
---|---|---|
D100N | lacks most of the bound cofactor NAD(H) and has lost all coenzyme NAD+-dependent MDH activity | Bacillus methanolicus |
D88N | only minor differences to wild-type in kinetics | Bacillus methanolicus |
G15A | only minor differences to wild-type in kinetics | Bacillus methanolicus |
G95A | impaired in cofactor NAD(H) binding but retains coenzyme NAD+-dependent MDH activity | Bacillus methanolicus |
G95A | low MDH activity, 10fold decrease in affinity for NAD+/NADH, insensitive to activator protein | Bacillus methanolicus |
K103R | lacks most of the bound cofactor NAD(H) and has lost all coenzyme NAD+-dependent MDH activity | Bacillus methanolicus |
S97G | impaired in cofactor NAD(H) binding but retains coenzyme NAD+-dependent MDH activity | Bacillus methanolicus |
S97G | insensitive to activator protein, but displays fully activated MDH reaction rates | Bacillus methanolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value of wild-type for NAD+ 0.04 mM, for NADH 0.01 mM, in absence of Mg2+, and for NAD+ 0.03 mM, for NADH 0.011 mM, in presence of Mg2+, of respectively | Bacillus methanolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bacillus methanolicus | |
Zn2+ | - |
Bacillus methanolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
10 * 43000, SDS-PAGE | Bacillus methanolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus methanolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Bacillus methanolicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
methanol + acceptor = formaldehyde + reduced acceptor | Ping-Pong type of reaction mechanism | Bacillus methanolicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.413 | - |
pH 9.5, 50°C | Bacillus methanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
Bacillus methanolicus | methanol + N,N-dimethyl-4-nitrosoaniline | - |
r | |
methanol + N,N-dimethyl-4-nitrosoaniline | - |
Bacillus methanolicus | formaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline | - |
r |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 43000, SDS-PAGE | Bacillus methanolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus methanolicus | |
NADH | - |
Bacillus methanolicus |