Literature summary for 1.1.99.37 extracted from

Vonck, J.; Arfman, N.; De Vries, G.E.; Van Beeumen, J.; Van Bruggen, E.F.J.; Dijkhuizen, L.
Electron microscopic analysis and biochemical characterization of a novel methanol dehydrogenase from the thermotolerant Bacillus sp. C1 (1991), J. Biol. Chem., 266, 3949-3954.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme forms a decamer with 5fold symmetry, electron microscopy	Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	85% inhibition at 1 mM	Bacillus sp. (in: Bacteria)
EDTA	40% inhibition at 5 mM	Bacillus sp. (in: Bacteria)
iodoacetate	less than 10% inhibition at a concentration of 5 mM	Bacillus sp. (in: Bacteria)
N-ethylmaleimide	less than 10% inhibition at a concentration of 5 mM	Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	contains one zinc and two magnesium ions per subunit	Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43000	-	10 * 43000, electron microscopy	Bacillus sp. (in: Bacteria)
363000	-	or above, sedimentation equilibrium centrifugation	Bacillus sp. (in: Bacteria)

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	-	-	-
Bacillus sp. (in: Bacteria) C1	-	-	-

Storage Stability

Storage Stability	Organism
-20°C, no dissociation occurrs when the isolated protein is stored frozen	Bacillus sp. (in: Bacteria)
at room temperature or at 4 °C, it dissociats into smaller fragments, probably monomers or dimers	Bacillus sp. (in: Bacteria)

Subunits

Subunits	Comment	Organism
decamer	10 * 43000, electron microscopy	Bacillus sp. (in: Bacteria)

Synonyms

Synonyms	Comment	Organism
NAD-dependent methanol dehydrogenase	-	Bacillus sp. (in: Bacteria)

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Bacillus sp. (in: Bacteria)

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Release 2023.1 (January 2023)