Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | - |
Acinetobacter calcoaceticus | |
lactose | - |
Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | under conditions that avoid its masking by sugar-substrate inhibition as much as possible, negative kinetic cooperativity with respect to sugar substrate oxidation is observed. The cooperativity effect dramatically changes the performance of soluble GDH, as reflected by the V2 and K2 values for glucose in phosphate buffer being about 10-fold and 100-fold higher than the V1 and K1 values, respectively. Substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose | Acinetobacter calcoaceticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose | Acinetobacter calcoaceticus | |
Sr2+ | substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glucose + acceptor = D-glucono-1,5-lactone + reduced acceptor | ping-pong behaviour and double-substrate inhibition | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + oxidized N-methylphenazonium methyl sulfate | - |
Acinetobacter calcoaceticus | D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate | - |
? | |
D-glucose + oxidized N-methylphenazonium methyl sulfate | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate | - |
? | |
D-xylose + oxidized N-methylphenazonium methyl sulfate | - |
Acinetobacter calcoaceticus | D-xylono-1,5-lactone + reduced N-methylphenazonium methyl sulfate | - |
? | |
lactose + oxidized N-methylphenazonium methyl sulfate | - |
Acinetobacter calcoaceticus | 4-O-beta-D-galactopyranosyl-D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
61 | - |
lactose | pH 7.0, 20°C | Acinetobacter calcoaceticus | |
92 | - |
D-glucose | pH 7.0, 20°C | Acinetobacter calcoaceticus | |
156 | - |
D-glucose | enzyme containing Sr2+ instead of Ca2+, pH 7.0, 20°C | Acinetobacter calcoaceticus |