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Literature summary for 1.1.99.35 extracted from

  • Yoshida, H.; Araki, N.; Tomisaka, A.; Sode, K.
    Secretion of water soluble pyrroloquinoline quinone glucose dehydrogenase by recombinant Pichia pastoris (2002), Enzyme Microb. Technol., 30, 312-318.
No PubMed abstract available

Application

Application Comment Organism
synthesis expression of enzyme in Pichia pastoris using the Saccharomyces cerevisiae alpha-factor signal sequence for secretion. The productivity of secreted PQQGDH-B achieves 218 kU/liter, i.e. 43 mg/liter. The secreted PQQGDH-B in Pichia pastoris is glycosylated but shows similar enzymatic properties as compared with those of recombinant PQQGDH-B produced in Escherichia coli Acinetobacter calcoaceticus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Acinetobacter calcoaceticus

Protein Variants

Protein Variants Comment Organism
additional information expression in Pichia pastoris using the Saccharomyces cerevisiae alpha-factor signal sequence for secretion. The productivity of secreted PQQGDH-B achieves 218 kU/liter, i.e. 43 mg/liter. The secreted PQQGDH-B in Pichia pastoris is glycosylated but shows similar enzymatic properties as compared with those of recombinant PQQGDH-B produced in Escherichia coli Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17.5
-
D-glucose recombinant enzyme, expression in Pichia pastoris Acinetobacter calcoaceticus
17.5
-
lactose recombinant enzyme, expression in Pichia pastoris Acinetobacter calcoaceticus
18.9
-
lactose recombinant enzyme, expression in Escherichia coli Acinetobacter calcoaceticus
25
-
D-glucose recombinant enzyme, expression in Escherichia coli Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the secreted PQQGDH-B in Pichia pastoris is glycosylated but shows similar enzymatic properties as compared with those of recombinant PQQGDH-B produced in Escherichia coli Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + oxidized 2,6-dichlorophenol-indophenol
-
Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
-
?
lactose + oxidized 2,6-dichlorophenol-indophenol
-
Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenol-indophenol
-
?