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Literature summary for 1.1.99.35 extracted from

  • Sode, K.; Ootera, T.; Shirahane, M.; Witarto, A.B.; Igarashi, S.; Yoshida, H.
    Increasing the thermal stability of the water-soluble pyrroloquinoline quinone glucose dehydrogenase by single amino acid replacement (2000), Enzyme Microb. Technol., 26, 491-496.
    View publication on PubMed
Search for more literature for 1.1.99.35

Protein Variants

Protein Variants Comment Organism
S231C substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 3%, D-mannose 8%, D-allose 46%, 3-O-methyl-D-glucose 76%, D-galactose 14%, D-xylose 8%, D-lactose 69%, D-maltose 69%, respectively Acinetobacter calcoaceticus
S231D substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 9%, D-allose 38%, 3-O-methyl-D-glucose 71%, D-galactose 12%, D-xylose 8%, D-lactose 54%, D-maltose 38%, respectively Acinetobacter calcoaceticus
S231H substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 12%, D-allose 57%, 3-O-methyl-D-glucose 86%, D-galactose 17%, D-xylose 7%, D-lactose 56%, D-maltose 38%, respectively Acinetobacter calcoaceticus
S231K increase in thermal stability. Substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 10%, D-allose 43%, 3-O-methyl-D-glucose 82%, D-galactose 15%, D-xylose 5%, D-lactose 59%, D-maltose 70%, respectively Acinetobacter calcoaceticus
S231L substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 6%, D-mannose 13%, D-allose 62%, 3-O-methyl-D-glucose 105%, D-galactose 20%, D-xylose 12%, D-lactose 73%, D-maltose 76%, respectively Acinetobacter calcoaceticus
S231M substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 9%, D-allose 43%, 3-O-methyl-D-glucose 80%, D-galactose 10%, D-xylose 8%, D-lactose 56%, D-maltose 41%, respectively Acinetobacter calcoaceticus
S231N substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 12%, D-allose 61%, 3-O-methyl-D-glucose 109%, D-galactose 18%, D-xylose 15%, D-lactose 66%, D-maltose 51%, respectively Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
25
-
 D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
27
-
 D-glucose mutant S231K, pH 7.0 Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2977
-
 wild-type, pH 7.0, at 50 mM D-glucose Acinetobacter calcoaceticus
3313
-
 mutant S231K, pH 7.0, at 100 mM D-glucose Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol 4% of the activity with D-glucose Acinetobacter calcoaceticus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol 81% of the activity with D-glucose Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-allose + oxidized 2,6-dichlorophenolindophenol 47% of the activity with D-glucose Acinetobacter calcoaceticus D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-galactose + oxidized 2,6-dichlorophenolindophenol 11% of the activity with D-glucose Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-mannose + oxidized 2,6-dichlorophenolindophenol 13% of the activity with D-glucose Acinetobacter calcoaceticus D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-xylose + oxidized 2,6-dichlorophenolindophenol 7% of the activity with D-glucose Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
lactose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
maltose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 wild-type, 5 min, 50% loss of activity. Mutant S231K, 50% loss of activity after 41 min Acinetobacter calcoaceticus