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Literature summary for 1.1.99.31 extracted from

  • Dewanti, A.R.; Xu, Y.; Mitra, B.
    Esters of mandelic acid as substrates for (S)-mandelate dehydrogenase from Pseudomonas putida: implications for the reaction mechanism (2004), Biochemistry, 43, 1883-1890.
    View publication on PubMed
Search for more literature for 1.1.99.31

Protein Variants

Protein Variants Comment Organism
R277G reduced activity compared to the wild type enzyme, the mutation at Arg277 has no influence on the binding affinity of ester substrates Pseudomonas putida
R277K reduced activity compared to the wild type enzyme, the mutation at Arg277 has no influence on the binding affinity of ester substrates Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
(S)-1-phenyl-1,2-ethanediol weak inhibitor Pseudomonas putida
(S)-1-phenyl-2,2,2-trifluoroethanol competitive inhibitor Pseudomonas putida
(S)-1-phenyl-2-propen-1-ol
-
 Pseudomonas putida
(S)-1-phenyl-2-propyn-1-ol
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 (S)-Mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
2.7
-
 ethyl-(S)-mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
2.9
-
 ethyl-(S)-mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
3.7
-
 ethyl-(S)-mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
5.6
-
 (S)-Mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
7.4
-
 methyl-(S)-mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
10.3
-
 methyl-(S)-mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
11.3
-
 methyl-(S)-mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
17
-
 (S)-Mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P20932
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-mandelate + 2 ferricyanide
-
 Pseudomonas putida 2-oxo-2-phenylacetate + 2 ferrocyanide + 2 H+
-
 ?
ethyl-(S)-mandelate + acceptor
-
 Pseudomonas putida ?
-
 ?
methyl-(S)-mandelate + acceptor
-
 Pseudomonas putida ?
-
 ?
additional information (S)-mandelamide, (S)-1-phenyl-2-propen-1-ol, (S)-1-phenyl-1,2-ethanediol, (S)-1-phenyl-2-propyn-1-ol, (R,S)-pantoyllactone, and (S)-1-phenyl-2,2,2-trifluoroethanol are no substrates Pseudomonas putida ?
-
 ?

Synonyms

Synonyms Comment Organism
MDH
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
 (S)-Mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
0.3
-
 ethyl-(S)-mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
2.3
-
 methyl-(S)-mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
2.5
-
 methyl-(S)-mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
2.6
-
 ethyl-(S)-mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
66
-
 (S)-Mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
151
-
 methyl-(S)-mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
204
-
 ethyl-(S)-mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
360
-
 (S)-Mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FMN dependent Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6
-
 (S)-1-phenyl-2,2,2-trifluoroethanol wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida
53.8
-
 (S)-1-phenyl-1,2-ethanediol wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 Pseudomonas putida