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Literature summary for 1.1.99.31 extracted from

  • Xu, Y.; Mitra, B.
    A highly active, soluble mutant of the membrane-associated (S)-mandelate dehydrogenase from Pseudomonas putida (1999), Biochemistry, 38, 12367-12376.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach. This mutant is very similar to the wild-type membrane-bound enzyme in its spectroscopic properties, substrate specificity, catalytic activity, kinetic mechanism, and lack of reactivity toeards oxygen. It should prove to be a highly useful model for structural studies of MDH Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.085
-
(S)-Mandelate pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
0.14
-
Mandelate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
0.18
-
(S)-Mandelate pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, wild-type enzyme Pseudomonas putida
0.24
-
indoleglycolate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
0.29
-
2-Hydroxyoctanoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
0.49
-
2-hydroxyisocaproate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
0.64
-
3-indolelactate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
0.89
-
2-hydroxyhexanoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.3
-
3-Phenyllactate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
2.6
-
2-Hydroxyisovalerate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
3.2
-
2-hydroxyvalerate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
10.3
-
2-Hydroxy-3-butenoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
13.2
-
2-Hydroxybutyrate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
17.2
-
2-Hydroxy-3-butynoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated Pseudomonas putida 16020
-

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-2-hydroxy-3-butenoic acid + acceptor
-
Pseudomonas putida 2-oxo-3-butenoic acid + reduced acceptor
-
?
(R,S)-2-hydroxyisovalerate + acceptor
-
Pseudomonas putida 2-oxo-isovalerate + reduced acceptor
-
?
(S)-mandelate + acceptor
-
Pseudomonas putida 2-oxo-2-phenylacetate + reduced acceptor
-
?
2-hydroxy-3-butynoate + acceptor
-
Pseudomonas putida 2-oxo-3-butynoate + reduced acceptor
-
?
2-hydroxybutyrate + acceptor
-
Pseudomonas putida 2-oxobutyrate + reduced acceptor
-
?
2-hydroxyhexanoate + acceptor
-
Pseudomonas putida 2-oxohexanoate + reduced acceptor
-
?
2-hydroxyisocaproate + acceptor
-
Pseudomonas putida 2-oxoisocaproate + reduced acceptor
-
?
2-hydroxyoctanoate + acceptor
-
Pseudomonas putida 2-oxooctanoate + reduced acceptor
-
?
2-hydroxyvalerate + acceptor
-
Pseudomonas putida 2-oxovalerate + reduced acceptor
-
?
3-indolelactate + acceptor
-
Pseudomonas putida 3-(1H-indol-2-yl)-2-oxopropanoate + reduced acceptor
-
?
3-phenyllactate + acceptor
-
Pseudomonas putida 2-oxo-3-phenylpropanoate + reduced acceptor
-
?
4-chloromandelate + acceptor
-
Pseudomonas putida 2-oxo-2-(4-chlorophenyl)acetate + reduced acceptor
-
?
indoleglycolate + acceptor
-
Pseudomonas putida indol-2-yl(oxo)acetate + reduced acceptor
-
?
mandelate + acceptor
-
Pseudomonas putida 2-oxo-2-phenylacetate + reduced acceptor
-
?

Synonyms

Synonyms Comment Organism
MDH
-
Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
2-Hydroxyisovalerate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
0.1
-
2-Hydroxybutyrate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
0.48
-
2-hydroxyhexanoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
0.5
-
2-hydroxyvalerate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
0.5
-
2-Hydroxy-3-butenoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
0.87
-
3-Phenyllactate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
0.96
-
2-Hydroxyoctanoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
1.28
-
2-hydroxyisocaproate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
3.13
-
3-indolelactate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
3.85
-
3-indolelactate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
6.6
-
2-Hydroxy-3-butynoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
38.5
-
indoleglycolate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
195
-
Mandelate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
205
-
(S)-Mandelate pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach Pseudomonas putida
290
-
(S)-Mandelate pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, wild-type enzyme Pseudomonas putida