Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris cells | Leucoagaricus meleagris |
Protein Variants | Comment | Organism |
---|---|---|
H103A | the mutant shows a reduction in catalytic efficiency for glucose by a factor of 10 compared to the wild type enzyme | Leucoagaricus meleagris |
H512A | the mutant shows a reduction in catalytic efficiency for glucose by a factor of 100000 compared to the wild type enzyme | Leucoagaricus meleagris |
H556A | the mutant shows a reduction in catalytic efficiency for glucose by a factor of 100 compared to the wild type enzyme | Leucoagaricus meleagris |
H556N | the mutant shows a reduction in catalytic efficiency for glucose by a factor of 1000 compared to the wild type enzyme | Leucoagaricus meleagris |
Q392A | the mutant shows a reduction in catalytic efficiency for glucose by a factor of 10 compared to the wild type enzyme | Leucoagaricus meleagris |
V511F | the mutant shows a reduction in catalytic efficiency for glucose by a factor of 100 compared to the wild type enzyme | Leucoagaricus meleagris |
V511W | the mutant shows a reduction in catalytic efficiency for glucose by a factor of 10 compared to the wild type enzyme | Leucoagaricus meleagris |
Y510A | the mutant shows a reduction in catalytic efficiency for glucose by a factor of 100 compared to the wild type enzyme | Leucoagaricus meleagris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
x * 90000, SDS-PAGE | Leucoagaricus meleagris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucoagaricus meleagris | Q3L245 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | approximately 30% degree of glycosylation | Leucoagaricus meleagris |
Purification (Comment) | Organism |
---|---|
cross-flow filtration, hydrophobic interaction and immobilized metal affinity chromatography | Leucoagaricus meleagris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ferrocenium ion | - |
Leucoagaricus meleagris | 2-dehydro-D-glucose + ferrocene | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, SDS-PAGE | Leucoagaricus meleagris |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Leucoagaricus meleagris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Leucoagaricus meleagris |