Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Leucoagaricus meleagris |
Crystallization (Comment) | Organism |
---|---|
microseeding, using 50 mM KH2PO4, 5% (v/v) 2-methyl-2,4-pentanediol, and 20% (w/v) polyethylene glycol 8000 | Leucoagaricus meleagris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a pyranoside + acceptor | Leucoagaricus meleagris | - |
a 3-dehydropyranoside + reduced acceptor | 3-dehydropyranoside or 3,4-didehydropyranoside | ? | |
D-glucose + acceptor | Leucoagaricus meleagris | the enzyme oxidizes D-glucose at both the C2 and C3 position but the C2 position is the primary site of attack | 2-dehydro-D-glucose + reduced acceptor | - |
? | |
additional information | Leucoagaricus meleagris | O2 is a poor electron acceptor | ? | - |
? | |
pyranose + acceptor | Leucoagaricus meleagris | - |
2-dehydropyranose + reduced acceptor | 2-dehydropyranose or 1-dehydropyranose or 3-dehydropyranose or 2,3-didehydropyranose | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucoagaricus meleagris | Q3L245 | - |
- |
Purification (Comment) | Organism |
---|---|
hydrophobic interaction chromatography and anion exchange chromatography | Leucoagaricus meleagris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-fluoro-3-deoxy-D-glucose + acceptor | - |
Leucoagaricus meleagris | ? + reduced acceptor | - |
? | |
a pyranoside + acceptor | - |
Leucoagaricus meleagris | a 3-dehydropyranoside + reduced acceptor | 3-dehydropyranoside or 3,4-didehydropyranoside | ? | |
D-glucose + acceptor | the enzyme oxidizes D-glucose at both the C2 and C3 position but the C2 position is the primary site of attack | Leucoagaricus meleagris | 2-dehydro-D-glucose + reduced acceptor | - |
? | |
additional information | O2 is a poor electron acceptor | Leucoagaricus meleagris | ? | - |
? | |
pyranose + acceptor | - |
Leucoagaricus meleagris | 2-dehydropyranose + reduced acceptor | 2-dehydropyranose or 1-dehydropyranose or 3-dehydropyranose or 2,3-didehydropyranose | ? |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Leucoagaricus meleagris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Leucoagaricus meleagris |