Application | Comment | Organism |
---|---|---|
synthesis | PDH catalysis with 1,4-benzoquinone as an oxidant provides biocatalytic sugar chemistry with a new convenient tool for high yield production of 3-keto-oligosaccharides and 3-keto-glycosides | Leucoagaricus meleagris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucoagaricus meleagris | - |
- |
- |
Leucoagaricus meleagris CCBAS 907 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
65fold | Leucoagaricus meleagris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Leucoagaricus meleagris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.7 | - |
pH 4.5, 25°C, D-glucose and 1,4-benzoquinone as substrates | Leucoagaricus meleagris |
Storage Stability | Organism |
---|---|
20°C, at least 14 days, stable | Leucoagaricus meleagris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + 1,4-benzoquinone | quinone-dependent PDH oxidizes alpha,alpha-trehalose at C-3 and C-3Â’, 5% of the activity with D-glucose | Leucoagaricus meleagris | 3,3'-didehydro-alpha,alpha-trehalose + hydroquinone | with 3-dehydro-alpha,alpha-trehalose as reaction intermediate | ? | |
alpha,alpha-trehalose + 1,4-benzoquinone | quinone-dependent PDH oxidizes alpha,alpha-trehalose at C-3 and C-3Â’, 5% of the activity with D-glucose | Leucoagaricus meleagris CCBAS 907 | 3,3'-didehydro-alpha,alpha-trehalose + hydroquinone | with 3-dehydro-alpha,alpha-trehalose as reaction intermediate | ? | |
D-glucose + 1,4-benzoquinone | simultaneous C-2 and C-3 oxidation of free D-glucose | Leucoagaricus meleagris | 3-dehydro-D-glucose + hydroquinone | or 2-dehydro-D-glucose | ? | |
D-glucose + 1,4-benzoquinone | simultaneous C-2 and C-3 oxidation of free D-glucose | Leucoagaricus meleagris CCBAS 907 | 3-dehydro-D-glucose + hydroquinone | or 2-dehydro-D-glucose | ? | |
erlose + 1,4-benzoquinone | quinone-dependent PDH oxidizes erlose at exclusively C-3 of its terminal glucopyranosyl moiety, 9% of the activity with D-glucose | Leucoagaricus meleagris | 3-dehydroerlose + hydroquinone | - |
? | |
erlose + 1,4-benzoquinone | quinone-dependent PDH oxidizes erlose at exclusively C-3 of its terminal glucopyranosyl moiety, 9% of the activity with D-glucose | Leucoagaricus meleagris CCBAS 907 | 3-dehydroerlose + hydroquinone | - |
? | |
melizitose + 1,4-benzoquinone | quinone-dependent PDH oxidizes melezitose exclusively at C-3 of its terminal glucopyranosyl moiety, 7% of the activity with D-glucose | Leucoagaricus meleagris | 3-dehydromelizitose + hydroquinone | - |
? | |
melizitose + 1,4-benzoquinone | quinone-dependent PDH oxidizes melezitose exclusively at C-3 of its terminal glucopyranosyl moiety, 7% of the activity with D-glucose | Leucoagaricus meleagris CCBAS 907 | 3-dehydromelizitose + hydroquinone | - |
? | |
methyl-alpha-D-galactopyranoside + 1,4-benzoquinone | formation of the C-3 carbonyl derivative, 7% of the activity with D-glucose | Leucoagaricus meleagris | methyl-alpha-3-dehydro-D-galactopyranoside + hydroquinone | - |
? | |
methyl-alpha-D-glucopyranoside + 1,4-benzoquinone | formation of the C-3 carbonyl derivative, 126% of the activity with D-glucose | Leucoagaricus meleagris | methyl-alpha-3-dehydro-D-glucopyranoside + hydroquinone | - |
? | |
methyl-beta-D-glucopyranoside + 1,4-benzoquinone | formation of the C-3 carbonyl derivative, 20% of the activity with D-glucose | Leucoagaricus meleagris | methyl-beta-3-dehydro-D-glucopyranoside + hydroquinone | - |
? | |
additional information | extremely broad substrate tolerance | Leucoagaricus meleagris | ? | - |
? | |
additional information | extremely broad substrate tolerance | Leucoagaricus meleagris CCBAS 907 | ? | - |
? | |
sucrose + 1,4-benzoquinone | quinone-dependent PDH oxidizes sucrose exclusively at C-3 of its terminal glucopyranosyl moiety, 11% of the activity with D-glucose | Leucoagaricus meleagris | 3'-dehydrosucrose + hydroquinone | oxidation of sucrose at C-3 of its terminal glucopyranosyl moiety | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Leucoagaricus meleagris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Leucoagaricus meleagris |