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Literature summary for 1.1.99.28 extracted from

  • Wiegert, T.; Sahm, H.; Sprenger, G.A.
    Export of the periplasmic NADP-containing glucose-fructose oxidoreductase of Zymomonas mobilis (1996), Arch. Microbiol., 166, 32-41.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
DELTA32-46 the mutant enzyme DELTA32-46 is a protein that is no longer processed but shows full enzymatic activity and has the cofactor firmly bound. The mutant enzyme DELTA2-20 or a mutant enzyme with an exchange of the entire signal sequence with the signal sequence of gluconolactonase of Zymomonas mobilis leads to an active and processed protein Zymomonas mobilis

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Zymomonas mobilis
-
-

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzyme DELTA32-46 Zymomonas mobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + D-fructose
-
Zymomonas mobilis D-glucono-1,5-lactone + D-sorbitol
-
?

Cofactor

Cofactor Comment Organism Structure
NADP+ tightly bound as hydrogen carrier Zymomonas mobilis
NADPH tightly bound as hydrogen carrier Zymomonas mobilis