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Literature summary for 1.1.99.28 extracted from
- Glucose-fructose oxidoreductase, a new enzyme isolated from Zymomonas mobilis that is responsible for sorbitol production (1986), J. Bacteriol., 167, 863-869.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Zymomonas mobilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | 16% inhibition at 2 M, 48% inhibition at 4.7 M | Zymomonas mobilis |  |
| sorbitol | 0.8 M, 27% inhibition | Zymomonas mobilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * 40000, at pH 5, SDS-PAGE | Zymomonas mobilis |
| 170000 | - |
gel filtration at pH 5.0 | Zymomonas mobilis |
| 300000 | - |
gel filtration at pH 7.0 | Zymomonas mobilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Glucose + D-fructose | Zymomonas mobilis | enzyme is responsible for sorbitol production | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zymomonas mobilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zymomonas mobilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 200 | - |
- |
Zymomonas mobilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Deoxy-D-glucose + D-fructose | at 6% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
| D-Galactose + D-fructose | at 8% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
| D-glucose + D-fructose | - |
Zymomonas mobilis | D-glucono-1,5-lactone + D-sorbitol | - |
? | |
| D-Glucose + D-fructose | enzyme is responsible for sorbitol production | Zymomonas mobilis | ? | - |
? | |
| D-Glucose + D-xylulose | at 7% of the activity relative to fructose | Zymomonas mobilis | ? | - |
? | |
| D-Glucose + dihydroxyacetone | at 6% of the activity relative to fructose | Zymomonas mobilis | ? | - |
? | |
| D-Glucose + L-sorbose | at 0.5% of the activity relative to fructose | Zymomonas mobilis | ? | - |
? | |
| D-Mannose + D-fructose | at 12% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
| D-Xylose + D-fructose | at 8% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
| DL-Glyceraldehyde + D-fructose | at 1.5% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
| L-Arabinose + D-fructose | at 3% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 40000, at pH 5, SDS-PAGE | Zymomonas mobilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
sorbitol | - |
Zymomonas mobilis | |
| 0.8 | - |
gluconolactone | - |
Zymomonas mobilis | |
| 200 | - |
D-glucose | - |
Zymomonas mobilis | |
| 200 | - |
D-fructose | - |
Zymomonas mobilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
- |
Zymomonas mobilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.1 | pH 5.0: about 35% of maximal activity, pH 7.1: about 30% of maximal activity | Zymomonas mobilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | tightly bound as hydrogen carrier | Zymomonas mobilis | |
| NADPH | tightly bound as hydrogen carrier | Zymomonas mobilis | |
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