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Literature summary for 1.1.99.20 extracted from

  • Kawai, F.; Kimura, T.; Yoshiki, T.; Yamada, H.; Mamoru, K.
    Purification and characterization of polyethylene glycol dehydrogenase involved in the bacterial metabolism of polyethylene glycol (1980), Appl. Environ. Microbiol., 40, 701-705.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
Flavobacterium sp.
Cd2+
-
Pseudomonas sp.
Co2+
-
Flavobacterium sp.
Co2+
-
Pseudomonas sp.
Cu2+ at 1 mM Flavobacterium sp.
Cu2+ at 1 mM Pseudomonas sp.
Fe3+
-
Flavobacterium sp.
Fe3+
-
Pseudomonas sp.
Hg2+ at 1 mM Flavobacterium sp.
Hg2+ at 1 mM Pseudomonas sp.
Mn2+
-
Flavobacterium sp.
Mn2+
-
Pseudomonas sp.
monoiodoacetate
-
Flavobacterium sp.
monoiodoacetate
-
Pseudomonas sp.
Ni2+
-
Flavobacterium sp.
Ni2+
-
Pseudomonas sp.
p-chloromercuribenzoate no inhibition with 2-mercaptoethanol Flavobacterium sp.
p-chloromercuribenzoate no inhibition with 2-mercaptoethanol Pseudomonas sp.
Pb2+
-
Flavobacterium sp.
Pb2+
-
Pseudomonas sp.
Zn2+
-
Flavobacterium sp.
Zn2+
-
Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
PEG-6000
-
Pseudomonas sp.
3
-
PEG-6000
-
Flavobacterium sp.
10
-
Tetraethylene glycol
-
Pseudomonas sp.
10
-
Tetraethylene glycol
-
Flavobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion requirement Pseudomonas sp.
additional information no metal ion requirement Flavobacterium sp.
potassium phosphate
-
Pseudomonas sp.
potassium phosphate
-
Flavobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
4 * 60000, mixed culture of Flavobacterium sp. and Pseudomonas sp., SDS-PAGE Pseudomonas sp.
60000
-
4 * 60000, mixed culture of Flavobacterium sp. and Pseudomonas sp., SDS-PAGE Flavobacterium sp.
240000
-
mixed culture of Flavobacterium sp. and Pseudomonas sp., gel filtration Pseudomonas sp.
240000
-
mixed culture of Flavobacterium sp. and Pseudomonas sp., gel filtration Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
synergistic mixed culture with Pseudomonas sp.
-
Pseudomonas sp.
-
synergistic mixed culture with Flavobacterium sp.
-

Purification (Commentary)

Purification (Comment) Organism
mixed culture, using precipitation with ammonium sulfate, solubilization with laurylbetaine and chromatography with diethylamino-ethyl-cellulose, hydroxylapatite and Sephadex G-200 Pseudomonas sp.
mixed culture, using precipitation with ammonium sulfate, solubilization with laurylbetaine and chromatography with diethylamino-ethyl-cellulose, hydroxylapatite and Sephadex G-200 Flavobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
mixed culture Pseudomonas sp.
1.2
-
mixed culture Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-heptanol + acceptor
-
Pseudomonas sp. n-heptanal + reduced acceptor
-
?
1-heptanol + acceptor
-
Flavobacterium sp. n-heptanal + reduced acceptor
-
?
1-hexanol + acceptor
-
Pseudomonas sp. n-hexanal + reduced acceptor
-
?
1-hexanol + acceptor
-
Flavobacterium sp. n-hexanal + reduced acceptor
-
?
diols + acceptor except ethylene glycol, 1,2-and 1,3-propenediol Pseudomonas sp. dialdehydes + reduced acceptor
-
?
diols + acceptor except ethylene glycol, 1,2-and 1,3-propenediol Flavobacterium sp. dialdehydes + reduced acceptor
-
?
diols + acceptor diethylene glycol and triethylene glycol are only poor substrates Pseudomonas sp. dialdehydes + reduced acceptor
-
?
diols + acceptor diethylene glycol and triethylene glycol are only poor substrates Flavobacterium sp. dialdehydes + reduced acceptor
-
?
PEG-1000 + acceptor
-
Pseudomonas sp. ?
-
?
PEG-1000 + acceptor
-
Flavobacterium sp. ?
-
?
PEG-20000 + acceptor
-
Pseudomonas sp. ?
-
?
PEG-20000 + acceptor
-
Flavobacterium sp. ?
-
?
PEG-400 + acceptor
-
Pseudomonas sp. ?
-
?
PEG-400 + acceptor
-
Flavobacterium sp. ?
-
?
PEG-4000 + acceptor
-
Pseudomonas sp. ?
-
?
PEG-4000 + acceptor
-
Flavobacterium sp. ?
-
?
PEG-6000 + acceptor most active substrate of all PEGs Pseudomonas sp. ?
-
?
PEG-6000 + acceptor most active substrate of all PEGs Flavobacterium sp. ?
-
?
polyethylene glycol + acceptor first and main step in aerobic PEG-metabolism Pseudomonas sp. ?
-
?
polyethylene glycol + acceptor first and main step in aerobic PEG-metabolism Flavobacterium sp. ?
-
?
primary alcohol + acceptor ethanol is only a poor substrate Pseudomonas sp. aldehyde + reduced acceptor
-
?
primary alcohol + acceptor ethanol is only a poor substrate Flavobacterium sp. aldehyde + reduced acceptor
-
?
primary alcohol + acceptor methanol, glycerol, secondary alcohols and sugar alcohols are no substrates Pseudomonas sp. aldehyde + reduced acceptor
-
?
primary alcohol + acceptor methanol, glycerol, secondary alcohols and sugar alcohols are no substrates Flavobacterium sp. aldehyde + reduced acceptor
-
?
primary alcohol + acceptor 2,6-dichlorophenolindophenol is the acceptor in vitro Pseudomonas sp. aldehyde + reduced acceptor
-
?
primary alcohol + acceptor 2,6-dichlorophenolindophenol is the acceptor in vitro Flavobacterium sp. aldehyde + reduced acceptor
-
?
tetraethylene glycol + acceptor
-
Pseudomonas sp. tetraethylene glycol aldehyde + reduced acceptor
-
?
tetraethylene glycol + acceptor
-
Flavobacterium sp. tetraethylene glycol aldehyde + reduced acceptor
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 60000, mixed culture of Flavobacterium sp. and Pseudomonas sp., SDS-PAGE Pseudomonas sp.
tetramer 4 * 60000, mixed culture of Flavobacterium sp. and Pseudomonas sp., SDS-PAGE Flavobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Pseudomonas sp.
60
-
-
Flavobacterium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 70 66% of maximal activity at 35°C and 70°C Pseudomonas sp.
35 70 66% of maximal activity at 35°C and 70°C Flavobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
stable below Pseudomonas sp.
35
-
stable below Flavobacterium sp.
60
-
94% loss of activity after 15 min at pH 8.0, the inicial velocity of 2,6-dichlorophenolindophenol reduction increases at temperatures up to around 60°C and rapidly declines at those above 60°C Pseudomonas sp.
60
-
94% loss of activity after 15 min at pH 8.0, the inicial velocity of 2,6-dichlorophenolindophenol reduction increases at temperatures up to around 60°C and rapidly declines at those above 60°C Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
toward PEG-6000 at a concentration of 5 mM Pseudomonas sp.
8
-
toward PEG-6000 at a concentration of 5 mM Flavobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 9 about half-maximal activity at pH 7.0 and 9.0 Pseudomonas sp.
7 9 about half-maximal activity at pH 7.0 and 9.0 Flavobacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
40% loss of activity at 5°C after 12 h Pseudomonas sp.
7
-
40% loss of activity at 5°C after 12 h Flavobacterium sp.
7.5 9 stable at 5°C for 12 h Pseudomonas sp.
7.5 9 stable at 5°C for 12 h Flavobacterium sp.
10
-
40% loss of activity at 5°C after 12 h Pseudomonas sp.
10
-
40% loss of activity at 5°C after 12 h Flavobacterium sp.

Cofactor

Cofactor Comment Organism Structure
2,6-dichlorophenolindophenol electron acceptor Pseudomonas sp.
2,6-dichlorophenolindophenol electron acceptor Flavobacterium sp.