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Literature summary for 1.1.99.18 extracted from
- Cellobiose dehydrogenase production by the mycelial culture of the mushroom Termitomyces clypeatus (2008), Process Biochem., 43, 634-641.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01861 | - |
cytochrome c | - |
Termitomyces clypeatus |  |
| 0.02253 | - |
2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | |
| 0.1379 | - |
ferricyanide | - |
Termitomyces clypeatus | |
| 0.181 | - |
cellobiose | - |
Termitomyces clypeatus | |
| 0.1905 | - |
cellotriose | - |
Termitomyces clypeatus | |
| 0.2105 | - |
cellotetraose | - |
Termitomyces clypeatus | |
| 0.2222 | - |
cellopentaose | - |
Termitomyces clypeatus | |
| 0.3846 | - |
alpha-lactose | - |
Termitomyces clypeatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
gel filtration | Termitomyces clypeatus |
| 101000 | - |
SDS-PAGE | Termitomyces clypeatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Termitomyces clypeatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ultrafiltration, gel filtration, hydrophobic interaction chromatography, gel filtration | Termitomyces clypeatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.76 | - |
- |
Termitomyces clypeatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-lactose + 2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | ? | - |
? | |
| cellobiose + 2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | ? | - |
? | |
| cellobiose + cytochrome c | - |
Termitomyces clypeatus | ? | - |
? | |
| cellobiose + ferricyanide | - |
Termitomyces clypeatus | ? | - |
? | |
| cellopentaose + 2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | ? | - |
? | |
| cellotetraose + 2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | ? | - |
? | |
| cellotriose + 2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | ? | - |
? | |
| lactose + H2O + acceptor | - |
Termitomyces clypeatus | lactobionic acid + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 100000, gel filtration, 1 * 101000, SDS-PAGE | Termitomyces clypeatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cellobiose dehydrogenase | - |
Termitomyces clypeatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
pH 5.0 | Termitomyces clypeatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
50% of activity is lost when incubated at 50°C for 30 min at pH 5.0 | Termitomyces clypeatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.88 | - |
cellotriose | - |
Termitomyces clypeatus | |
| 8.88 | - |
cellotetraose | - |
Termitomyces clypeatus | |
| 9.31 | - |
cellopentaose | - |
Termitomyces clypeatus | |
| 14.22 | - |
alpha-lactose | - |
Termitomyces clypeatus | |
| 16.01 | - |
2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | |
| 17.65 | - |
cellobiose | - |
Termitomyces clypeatus | |
| 19.69 | - |
cytochrome c | - |
Termitomyces clypeatus | |
| 31.36 | - |
ferricyanide | - |
Termitomyces clypeatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | depending on the electron acceptor | Termitomyces clypeatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 8 | at least for 12 h, assayed with 2,6-dichlorophenol indophenol | Termitomyces clypeatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Termitomyces clypeatus | |
| heme | - |
Termitomyces clypeatus | |
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