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Literature summary for 1.1.99.18 extracted from

  • Harreither, W.; Sygmund, C.; Duenhofen, E.; Vicuna, R.; Haltrich, D.; Ludwig, R.
    Cellobiose dehydrogenase from the ligninolytic basidiomycete Ceriporiopsis subvermispora (2009), Appl. Environ. Microbiol., 75, 2750-2757.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.18

Cloned(Commentary)

Cloned (Comment) Organism
-
 Gelatoporia subvermispora

Inhibitors

Inhibitors Comment Organism Structure
cellobiose substrate inhibition Gelatoporia subvermispora
cytochrome c substrate inhibition Gelatoporia subvermispora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00032
-
 ferricenium cellobiose as substrate, pH 3.5 Gelatoporia subvermispora
0.00094
-
 cytochrome c cellobiose as substrate, pH 3.5 Gelatoporia subvermispora
0.011
-
 ferricyanide cellobiose as substrate, pH 3.5 Gelatoporia subvermispora
0.012
-
 2,6-dichloroindophenol cellobiose as substrate, pH 4.5 Gelatoporia subvermispora
0.026
-
 1,4-benzoquinone cellobiose as substrate, pH 3.5 Gelatoporia subvermispora
0.082
-
 cellobiose cytochrome c as electron acceptor Gelatoporia subvermispora
0.14
-
 cellobiose 2,6-dichloroindophenol as electron acceptor Gelatoporia subvermispora
4.3
-
 lactose cytochrome c as electron acceptor Gelatoporia subvermispora
4.4
-
 lactose 2,6-dichloroindophenol as electron acceptor Gelatoporia subvermispora
280
-
 maltose cytochrome c as electron acceptor Gelatoporia subvermispora
300
-
 maltose 2,6-dichloroindophenol as electron acceptor Gelatoporia subvermispora
3300
-
 D-glucose 2,6-dichloroindophenol as electron acceptor Gelatoporia subvermispora
3800
-
 D-glucose cytochrome c as electron acceptor Gelatoporia subvermispora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 x * 84000 (PNGase treated), two subunit-glycoforms: 98000 and 87000, SDS-PAGE Gelatoporia subvermispora

Organism

Organism UniProt Comment Textmining
Gelatoporia subvermispora B6D1P2
-
-
Gelatoporia subvermispora FP-90031 B6D1P2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 2 glycoforms Gelatoporia subvermispora

Purification (Commentary)

Purification (Comment) Organism
diafiltration, ion exchange chromatography, hydrophobic interaction chromatography, ion exchange chromatography Gelatoporia subvermispora

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.3
-
-
 Gelatoporia subvermispora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichloroindophenol
-
 Gelatoporia subvermispora ?
-
 ?
cellobiose + cytochrome c
-
 Gelatoporia subvermispora ?
-
 ?
D-glucose + 2,6-dichloroindophenol
-
 Gelatoporia subvermispora ?
-
 ?
D-glucose + 2,6-dichloroindophenol
-
 Gelatoporia subvermispora FP-90031 ?
-
 ?
D-glucose + cytochrome c
-
 Gelatoporia subvermispora ?
-
 ?
lactose + 1,4-benzoquinone
-
 Gelatoporia subvermispora ?
-
 ?
lactose + 1,4-benzoquinone
-
 Gelatoporia subvermispora FP-90031 ?
-
 ?
lactose + 2,6-dichloroindophenol
-
 Gelatoporia subvermispora ?
-
 ?
lactose + 2,6-dichloroindophenol
-
 Gelatoporia subvermispora FP-90031 ?
-
 ?
lactose + cytochrome c
-
 Gelatoporia subvermispora ?
-
 ?
lactose + cytochrome c
-
 Gelatoporia subvermispora FP-90031 ?
-
 ?
lactose + ferricenium
-
 Gelatoporia subvermispora ?
-
 ?
lactose + ferricyanide
-
 Gelatoporia subvermispora ?
-
 ?
maltose + 2,6-dichloroindophenol
-
 Gelatoporia subvermispora ?
-
 ?
maltose + 2,6-dichloroindophenol
-
 Gelatoporia subvermispora FP-90031 ?
-
 ?
maltose + cytochrome c
-
 Gelatoporia subvermispora ?
-
 ?

Subunits

Subunits Comment Organism
? x * 84000 (PNGase treated), two subunit-glycoforms: 98000 and 87000, SDS-PAGE Gelatoporia subvermispora

Synonyms

Synonyms Comment Organism
cellobiose (acceptor) 1-oxidoreductase
-
 Gelatoporia subvermispora
cellobiose dehydrogenase
-
 Gelatoporia subvermispora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 broad optimum: 30°C-40°C, lactose as substrate, cytochrome c as electron acceptor, pH 3.5 Gelatoporia subvermispora
60
-
 lactose as substrate, 2,6-dichloroindophenol as electron acceptor, pH 4.5 Gelatoporia subvermispora

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 lactose as substrate, cytochrome c as electron acceptor, pH 3.5 (analysis starts at 20°C) Gelatoporia subvermispora
20 70 lactose as substrate, 2,6-dichloroindophenol as electron acceptor, pH 4.5 (analysis starts at 20°C) Gelatoporia subvermispora

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.91
-
 maltose cytochrome c as electron acceptor Gelatoporia subvermispora
1.03
-
 maltose 2,6-dichloroindophenol as electron acceptor Gelatoporia subvermispora
2 8 lactose 2,6-dichloroindophenol as electron acceptor Gelatoporia subvermispora
3.4
-
 D-glucose cytochrome c as electron acceptor Gelatoporia subvermispora
3.8
-
 D-glucose 2,6-dichloroindophenol as electron acceptor Gelatoporia subvermispora
25
-
 cellobiose 2,6-dichloroindophenol as electron acceptor Gelatoporia subvermispora
26
-
 cellobiose cytochrome c as electron acceptor Gelatoporia subvermispora
27
-
 lactose cytochrome c as electron acceptor Gelatoporia subvermispora
29
-
 2,6-dichloroindophenol cellobiose as substrate, pH 4.5 Gelatoporia subvermispora
45
-
 1,4-benzoquinone cellobiose as substrate, pH 3.5 Gelatoporia subvermispora
58
-
 ferricenium cellobiose as substrate, pH 3.5 Gelatoporia subvermispora
60
-
 cytochrome c cellobiose as substrate, pH 3.5 Gelatoporia subvermispora
61
-
 ferricyanide cellobiose as substrate, pH 3.5 Gelatoporia subvermispora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 lactose as substrate, 1,4-benzoquinone or ferricenium or cytochrome c as electron acceptor Gelatoporia subvermispora
4.5
-
 lactose as substrate, 2,6-dichloroindophenol as electron acceptor Gelatoporia subvermispora

pH Range

pH Minimum pH Maximum Comment Organism
2.5 6.5 lactose as substrate, 2,6-dichloroindophenol or 1,4-benzoquinone or ferricenium as electron acceptor Gelatoporia subvermispora
2.5 4.5 lactose as substrate, cytochrome c as electron acceptor Gelatoporia subvermispora

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Gelatoporia subvermispora
heme
-
 Gelatoporia subvermispora

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.195
-
 cytochrome c cellobiose as substrate, pH 3.5 Gelatoporia subvermispora
125
-
 cellobiose 2,6-dichloroindophenol as electron acceptor Gelatoporia subvermispora
150
-
 cellobiose cytochrome c as electron acceptor Gelatoporia subvermispora

pI Value

Organism Comment pI Value Maximum pI Value
Gelatoporia subvermispora one of the two glycoforms, calculated: 4.2
-
 3
Gelatoporia subvermispora one of the two glycoforms, calculated: 4.2
-
 3.1