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Literature summary for 1.1.99.18 extracted from

  • Zamocky, M.; Schuemann, C.; Sygmund, C.; OCallaghan, J.; Dobson, A.D.; Ludwig, R.; Haltrich, D.; Peterbauer, C.K.
    Cloning, sequence analysis and heterologous expression in Pichia pastoris of a gene encoding a thermostable cellobiose dehydrogenase from Myriococcum thermophilum (2008), Protein Expr. Purif., 59, 258-265.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Thermothelomyces myriococcoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
cellobiose 2,6-dichloroindophenol as electron acceptor, at 40°C Thermothelomyces myriococcoides
0.14
-
lactose 2,6-diclorindophenol as electron acceptor, at 40°C Thermothelomyces myriococcoides
8.86
-
maltose 2,6-diclorindophenol as electron acceptor, at 40°C Thermothelomyces myriococcoides
21.5
-
cytochrome c lactose as electron donor, at 40°C Thermothelomyces myriococcoides
140.4
-
2,6-dichloroindophenol lactose as electron donor, at 40°C Thermothelomyces myriococcoides
157.1
-
glucose 2,6-diclorindophenol as electron acceptor, at 40°C Thermothelomyces myriococcoides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
SDS-PAGE Thermothelomyces myriococcoides

Organism

Organism UniProt Comment Textmining
Thermothelomyces myriococcoides
-
-
-
Thermothelomyces myriococcoides CBS 208.89
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by hi-trap column and phenyl HIC column chromatography, deglycosylation Thermothelomyces myriococcoides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.4
-
HIC-column purified Thermothelomyces myriococcoides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichloroindophenol
-
Thermothelomyces myriococcoides cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
cellobiose + 2,6-dichloroindophenol
-
Thermothelomyces myriococcoides CBS 208.89 cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
D-glucose + 2,6-dichloroindophenol
-
Thermothelomyces myriococcoides ?
-
?
D-glucose + 2,6-dichloroindophenol
-
Thermothelomyces myriococcoides CBS 208.89 ?
-
?
lactose + 2,6-dichloroindophenol
-
Thermothelomyces myriococcoides ?
-
?
lactose + 2,6-dichloroindophenol
-
Thermothelomyces myriococcoides CBS 208.89 ?
-
?
lactose + cytochrome c
-
Thermothelomyces myriococcoides ?
-
?
lactose + cytochrome c
-
Thermothelomyces myriococcoides CBS 208.89 ?
-
?
maltose + 2,6-dichloroindophenol
-
Thermothelomyces myriococcoides ?
-
?
maltose + 2,6-dichloroindophenol
-
Thermothelomyces myriococcoides CBS 208.89 ?
-
?

Synonyms

Synonyms Comment Organism
cellobiose dehydrogenase CDH Thermothelomyces myriococcoides
MtCDH
-
Thermothelomyces myriococcoides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
lactose as electron donor, 2,6-diclorindophenol as electron acceptor Thermothelomyces myriococcoides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 55 stable for 50 h Thermothelomyces myriococcoides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
lactose as electron donor, 2,6-diclorindophenol as electron acceptor Thermothelomyces myriococcoides
5
-
lactose as electron donor, cytochrome c as electron acceptor Thermothelomyces myriococcoides

pH Range

pH Minimum pH Maximum Comment Organism
4 7 lactose as electron donor, 2,6-diclorindophenol as electron acceptor Thermothelomyces myriococcoides