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Literature summary for 1.1.99.18 extracted from

  • Nakagame, S.; Furujyo, A.; Sugiura, J.
    Purification and characterization of cellobiose dehydrogenase from white-rot basidiomycete Trametes hirsuta (2006), Biosci. Biotechnol. Biochem., 70, 1629-1635.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
cytochrome c electron acceptor Trametes hirsuta
0.0122
-
DCPIP electron acceptor Trametes hirsuta
0.0389
-
3,5-di-tert-butylbenzoquinone electron acceptor Trametes hirsuta
0.042
-
cellobiose electron donor Trametes hirsuta
0.214
-
K3Fe(CN)6 electron acceptor Trametes hirsuta
0.216
-
cellotriose electron donor Trametes hirsuta
1.396
-
cellotetraose electron donor Trametes hirsuta
2.126
-
lactose electron donor Trametes hirsuta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91700
-
estimated by SDS-PAGE analysis Trametes hirsuta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + acceptor Trametes hirsuta
-
cellobiono-1,5-lactone + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Trametes hirsuta
-
IFO 4917
-

Purification (Commentary)

Purification (Comment) Organism
using A POROS HP 2, gel filtation, a HI Load Superdex-200 and a Mono Q column Trametes hirsuta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.08
-
purification step culture filtration Trametes hirsuta
0.17
-
purification step ultra filtration Trametes hirsuta
1.79
-
purification step POROS HP2 Trametes hirsuta
2.64
-
purification step HiLoad Superdex-200 Trametes hirsuta
16.3
-
purification step Mono Q Trametes hirsuta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenolindophenol DCPIP, activity assay Trametes hirsuta cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
cellobiose + 3,5-di-tert-butylbenzoquinone
-
Trametes hirsuta cellobiono-1,5-lactone + reduced 3,5-di-tert-butylbenzoquinone
-
r
cellobiose + acceptor
-
Trametes hirsuta cellobiono-1,5-lactone + reduced acceptor
-
?
cellobiose + K3Fe(CN)6
-
Trametes hirsuta cellobiono-1,5-lactone + reduced K3Fe(CN)6
-
r
cellotetraose + acceptor
-
Trametes hirsuta cellotetraono-1,5-lactone + reduced acceptor
-
?
cellotriose + 2,6-dichlorophenolindophenol
-
Trametes hirsuta cellotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
r
lactose + acceptor
-
Trametes hirsuta 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
?
lactose + cytochrome c
-
Trametes hirsuta 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
?

Synonyms

Synonyms Comment Organism
Cdh
-
Trametes hirsuta
cellobiose dehydrogenase
-
Trametes hirsuta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
activity assay Trametes hirsuta

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 70
-
Trametes hirsuta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
very stable below 40°C, above 60°C all activity is lost after 30 min Trametes hirsuta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
activity assay Trametes hirsuta

pH Range

pH Minimum pH Maximum Comment Organism
4 7 at 40°C Trametes hirsuta

pI Value

Organism Comment pI Value Maximum pI Value
Trametes hirsuta
-
-
4.2