KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
cytochrome c | electron acceptor | Trametes hirsuta | |
0.0122 | - |
DCPIP | electron acceptor | Trametes hirsuta | |
0.0389 | - |
3,5-di-tert-butylbenzoquinone | electron acceptor | Trametes hirsuta | |
0.042 | - |
cellobiose | electron donor | Trametes hirsuta | |
0.214 | - |
K3Fe(CN)6 | electron acceptor | Trametes hirsuta | |
0.216 | - |
cellotriose | electron donor | Trametes hirsuta | |
1.396 | - |
cellotetraose | electron donor | Trametes hirsuta | |
2.126 | - |
lactose | electron donor | Trametes hirsuta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
91700 | - |
estimated by SDS-PAGE analysis | Trametes hirsuta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + acceptor | Trametes hirsuta | - |
cellobiono-1,5-lactone + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes hirsuta | - |
IFO 4917 | - |
Purification (Comment) | Organism |
---|---|
using A POROS HP 2, gel filtation, a HI Load Superdex-200 and a Mono Q column | Trametes hirsuta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
purification step culture filtration | Trametes hirsuta |
0.17 | - |
purification step ultra filtration | Trametes hirsuta |
1.79 | - |
purification step POROS HP2 | Trametes hirsuta |
2.64 | - |
purification step HiLoad Superdex-200 | Trametes hirsuta |
16.3 | - |
purification step Mono Q | Trametes hirsuta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichlorophenolindophenol | DCPIP, activity assay | Trametes hirsuta | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
cellobiose + 3,5-di-tert-butylbenzoquinone | - |
Trametes hirsuta | cellobiono-1,5-lactone + reduced 3,5-di-tert-butylbenzoquinone | - |
r | |
cellobiose + acceptor | - |
Trametes hirsuta | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
cellobiose + K3Fe(CN)6 | - |
Trametes hirsuta | cellobiono-1,5-lactone + reduced K3Fe(CN)6 | - |
r | |
cellotetraose + acceptor | - |
Trametes hirsuta | cellotetraono-1,5-lactone + reduced acceptor | - |
? | |
cellotriose + 2,6-dichlorophenolindophenol | - |
Trametes hirsuta | cellotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
r | |
lactose + acceptor | - |
Trametes hirsuta | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
lactose + cytochrome c | - |
Trametes hirsuta | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cdh | - |
Trametes hirsuta |
cellobiose dehydrogenase | - |
Trametes hirsuta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
activity assay | Trametes hirsuta |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | - |
Trametes hirsuta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
very stable below 40°C, above 60°C all activity is lost after 30 min | Trametes hirsuta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
activity assay | Trametes hirsuta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | at 40°C | Trametes hirsuta |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trametes hirsuta | - |
- |
4.2 |