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Literature summary for 1.1.99.18 extracted from

  • Rotsaert, F.A.J.; Hallberg, B.M.; de Vrise, S.; Moenne-Loccoz, P.; Divne, C.; Renganathan, V.; Gold, M.H.
    Biophysical and structural analysis of a novel heme b iron ligation in the flavocytochrome cellobiose dehydrogenase (2003), J. Biol. Chem., 278, 33224-33231.
    View publication on PubMed
Search for more literature for 1.1.99.18

Cloned(Commentary)

Cloned (Comment) Organism
transformation of Ura- strain Ura11 with linearized pM65H Phanerodontia chrysosporium

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion method, crystal structure of M65H cytochrome domain determined at 1.9 A resolution Phanerodontia chrysosporium

Protein Variants

Protein Variants Comment Organism
M65H the variant retains the flavin catalytic reactivity, the ability of the mutant to reduce external one-electron acceptors such as cytochrome c is impaired, decrease in the redox midpoint potential of the heme by 210 mV. IN contrast to the wild-type enzyme, the ferric state of the protoheme displays a mixed low spin/high spin state at room temperature and low spion character at 90 K Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 cytochrome c pH 4.5, reaction with cellobiose, wild-type enzyme Phanerodontia chrysosporium
0.0064
-
 2,6-dichlorophenol indophenol pH 4.5, reaction with cellobiose, wild-type enzyme Phanerodontia chrysosporium
0.0074
-
 2,6-dichlorophenol indophenol pH 4.5, reaction with cellobiose, mutant enzyme M65H Phanerodontia chrysosporium
0.018
-
 cellobiose pH 4.5, electron acceptor: cytochrome c, wild-type enzyme Phanerodontia chrysosporium
0.035
-
 cellobiose pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, mutant enzyme M65H Phanerodontia chrysosporium
0.04
-
 cellobiose pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, wild-type enzyme Phanerodontia chrysosporium

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Phanerodontia chrysosporium
-
-

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium Q01738 variant M65H
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenol indophenol
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
cellobiose + cytochrome c
-
 Phanerodontia chrysosporium cellobiono-1,5-lactone + reduced cytochrome c
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 cellobiose pH 4.5, electron acceptor: cytochrome c, mutant enzyme M65H Phanerodontia chrysosporium
0.1
-
 cytochrome c pH 4.5, reaction with cellobiose, mutant enzyme M65H Phanerodontia chrysosporium
10.5
-
 cellobiose pH 4.5, electron acceptor: cytochrome c, wild-type enzyme Phanerodontia chrysosporium
10.8
-
 cytochrome c pH 4.5, reaction with cellobiose, wild-type enzyme Phanerodontia chrysosporium
25
-
 2,6-dichlorophenol indophenol pH 4.5, reaction with cellobiose, mutant enzyme M65H Phanerodontia chrysosporium
25.7
-
 cellobiose pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, wild-type enzyme Phanerodontia chrysosporium
26
-
 cellobiose pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, mutant enzyme M65H Phanerodontia chrysosporium
27
-
 2,6-dichlorophenol indophenol pH 4.5, reaction with cellobiose, wild-type enzyme Phanerodontia chrysosporium

Cofactor

Cofactor Comment Organism Structure
flavin flavocytochrome Phanerodontia chrysosporium
heme flavocytochrome Phanerodontia chrysosporium