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Literature summary for 1.1.99.18 extracted from

  • Ayers, A.R.; Eriksson, K.E.
    Cellobiose oxidase from Sporotrichum pulverulentum (1982), Methods Enzymol., 89, 129-135.
    View publication on PubMed
Search for more literature for 1.1.99.18

Inhibitors

Inhibitors Comment Organism Structure
2,2-bipyridine
-
 Phanerodontia chrysosporium
Sodium azide
-
 Phanerodontia chrysosporium
Sodium cyanide
-
 Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93000
-
 sedimentation equilibrium centrifugation Phanerodontia chrysosporium
102000
-
 1 * 102000, SDS-PAGE Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + O2 Phanerodontia chrysosporium role in cellulose degradation cellobiono-1,5-lactone + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
 white-rot fungus
-
Phanerodontia chrysosporium
-
 strain ATCC 32629
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein binds to immobilized concanavalin A Phanerodontia chrysosporium

Purification (Commentary)

Purification (Comment) Organism
-
 Phanerodontia chrysosporium

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Phanerodontia chrysosporium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.417
-
-
 Phanerodontia chrysosporium

Storage Stability

Storage Stability Organism
-20°C, 50 mM sodium acetate buffer, pH 5, 1 week, less than 10% loss of activity Phanerodontia chrysosporium
0°C, 50 mM sodium acetate buffer, pH 5, 1 week, less than 10% loss of activity Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-beta-glucosylmannose + O2 slowly oxidized Phanerodontia chrysosporium ?
-
 ?
cellobiose + O2 enzyme consumes 1 mol molecular oxygen per mol of substrate oxidized Phanerodontia chrysosporium cellobiono-1,5-lactone + H2O2 reaction product is cellobionic acid, no H2O2 detected, but superoxide anion ?
cellobiose + O2 readily oxidized, cellobiose and cellohexaose with almost same activity Phanerodontia chrysosporium cellobiono-1,5-lactone + H2O2 reaction product is cellobionic acid, no H2O2 detected, but superoxide anion ?
cellobiose + O2 role in cellulose degradation Phanerodontia chrysosporium cellobiono-1,5-lactone + H2O2
-
 ?
cellodextrin + O2 decreasing rapidity in the series from cellobiose to cellotetraose, but then with increasing size they are more rapidly oxidized Phanerodontia chrysosporium aldonic acid + H2O2
-
 ?
galactose + O2 slowly oxidized Phanerodontia chrysosporium ?
-
 ?
lactose + O2 readily oxidized Phanerodontia chrysosporium 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + H2O2 product is lactobionic acid ?
mannose + O2 slowly oxidized Phanerodontia chrysosporium ?
-
 ?
additional information not as substrates: maltose, sophorose, glucose, xylose, cellulose Phanerodontia chrysosporium ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 102000, SDS-PAGE Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Phanerodontia chrysosporium

Cofactor

Cofactor Comment Organism Structure
flavin flavohemoprotein, one flavin per enzyme molecule Phanerodontia chrysosporium
heme b flavohemoprotein Phanerodontia chrysosporium