Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lactobiono-1,5-lactone | weak product inhibition | Athelia rolfsii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
cellobiose | electron acceptor cytochrome c from horse heart | Athelia rolfsii | |
0.0004 | - |
cellobiose | electron acceptor 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid) cation radical | Athelia rolfsii | |
0.002 | - |
cellobiose | electron acceptor K3Fe(CN)6 | Athelia rolfsii | |
0.0044 | - |
cellobiose | electron acceptors methylen green and blue | Athelia rolfsii | |
0.015 | - |
cellobiose | electron acceptor 2,6-dichloroindophenol | Athelia rolfsii | |
0.025 | - |
cellobiose | electron acceptor 1,4-benzoquinone | Athelia rolfsii | |
0.031 | - |
cellobiose | electron acceptor meldola blue | Athelia rolfsii | |
0.053 | - |
cellobiose | electron acceptor 3,5-di-tert-butyl-1,2-benzoquinone | Athelia rolfsii | |
0.12 | - |
cellobiose | - |
Athelia rolfsii | |
0.49 | - |
cellotriose | - |
Athelia rolfsii | |
0.54 | - |
cellopentaose | - |
Athelia rolfsii | |
0.55 | - |
cellobiose | electron acceptor 2,6-dimethyl-1,4-benzoquinone | Athelia rolfsii | |
0.6 | - |
cellotetraose | - |
Athelia rolfsii | |
2 | - |
beta-lactose | 70-75% | Athelia rolfsii | |
2.3 | - |
thiocellobiose | - |
Athelia rolfsii | |
2.4 | - |
lactose | - |
Athelia rolfsii | |
5.4 | - |
xylobiose | - |
Athelia rolfsii | |
54 | - |
alpha-lactose | - |
Athelia rolfsii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Athelia rolfsii | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
101000 | - |
MALDI-MS | Athelia rolfsii |
110000 | - |
1 * 110000, SDS-PAGE | Athelia rolfsii |
126000 | - |
native PAGE | Athelia rolfsii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Athelia rolfsii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE sepharose, Resource PHE | Athelia rolfsii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Athelia rolfsii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
63 | - |
electron acceptor 2,6-dichloroindophenol | Athelia rolfsii |
66 | - |
electron acceptor cytochrome c | Athelia rolfsii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-lactose + acceptor | - |
Athelia rolfsii | 4-O-(alpha-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
beta-lactose + acceptor | - |
Athelia rolfsii | 4-O-(beta-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
cellobiose + acceptor | artificial electron acceptors: 2,6-dichlorophenolindophenol, 1,4-benzoquinone, 2,6-dimethyl-1,4-benzoquinone, 2,6-dimethoxy-1,4-benzoquinone, 3,5-di-tert-butyl-1,2-benzoquinone, methylene blue and green, meldola blue, 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid), cytochrome c, K3Fe(CN)6 | Athelia rolfsii | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
cellopentaose + acceptor | - |
Athelia rolfsii | cellopentaono-1,5-lactone + reduced acceptor | - |
? | |
cellotetraose + acceptor | - |
Athelia rolfsii | cellotetrono-1,5-lactone + reduced acceptor | - |
? | |
cellotriose + acceptor | - |
Athelia rolfsii | cellotriono-1,5-lactone + reduced acceptor | - |
? | |
lactose + acceptor | - |
Athelia rolfsii | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
thiocellobiose + acceptor | - |
Athelia rolfsii | thiocellobiono-1,5-lactone + reduced acceptor | - |
? | |
xylobiose + 2,6-dichlorophenolindophenol | - |
Athelia rolfsii | 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 110000, SDS-PAGE | Athelia rolfsii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
cellobiose | electron acceptor 2,6-dimethoxy-1,4-benzoquinone | Athelia rolfsii | |
15 | - |
cellobiose | electron acceptor 2,6-dimethyl-1,4-benzoquinone | Athelia rolfsii | |
17 | - |
alpha-lactose | - |
Athelia rolfsii | |
18 | - |
cellobiose | electron acceptors methylene blue or methylene green | Athelia rolfsii | |
23 | - |
cellobiose | electron acceptor 3,5-di-tert-butyl-1,2-benzoquinone | Athelia rolfsii | |
24 | - |
cellotetraose | - |
Athelia rolfsii | |
24 | - |
cellopentaose | - |
Athelia rolfsii | |
24 | - |
cellobiose | electron acceptor meldola blue | Athelia rolfsii | |
26 | - |
lactose | - |
Athelia rolfsii | |
26 | - |
cellotriose | - |
Athelia rolfsii | |
27 | - |
cellobiose | - |
Athelia rolfsii | |
27 | - |
xylobiose | - |
Athelia rolfsii | |
27 | - |
thiocellobiose | - |
Athelia rolfsii | |
27 | - |
cellobiose | electron acceptor 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid) cation radical | Athelia rolfsii | |
30 | - |
cellobiose | 2,6-dichloroindophenpl or 1,4-benzoquinone as electron acceptor | Athelia rolfsii | |
34 | - |
cellobiose | electron acceptor cytochrome c from horseheart | Athelia rolfsii | |
37 | - |
cellobiose | electron acceptor K3Fe(CN)6 | Athelia rolfsii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.7 | 5 | depends on electron acceptor | Athelia rolfsii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.7 | - |
at 35°C, enzyme loses 50% of its initial activity after 10 min at pH 2.2, stability optimum shifts to 5.0 at 46°C with a half-life of 12 h | Athelia rolfsii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 heme per enzyme molecule, 1 FAD nucleotide per molecule | Athelia rolfsii | |
heme b | 1 heme per enzyme molecule, 1 FAD nucleotide per molecule | Athelia rolfsii | |
additional information | limited proteolytic cleavage of the enzyme leads to an inactive heme peptide and an active FAD domain which has been termed cellobiose: quinone 1-oxidoreductase EC 1.5.1.1 | Athelia rolfsii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
31 | - |
lactobiono-1,5-lactone | - |
Athelia rolfsii |