Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.99.18 extracted from

  • Baminger, U.; Subramaniam, S.S.; Renganathan, V.; Halterich, d.
    Purification and characterization of cellobiose dehydrogenase from the plant pathogen Sclerotium (Athelia) rolfsii (2001), Appl. Environ. Microbiol., 67, 1766-1774.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
lactobiono-1,5-lactone weak product inhibition Athelia rolfsii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
cellobiose electron acceptor cytochrome c from horse heart Athelia rolfsii
0.0004
-
cellobiose electron acceptor 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid) cation radical Athelia rolfsii
0.002
-
cellobiose electron acceptor K3Fe(CN)6 Athelia rolfsii
0.0044
-
cellobiose electron acceptors methylen green and blue Athelia rolfsii
0.015
-
cellobiose electron acceptor 2,6-dichloroindophenol Athelia rolfsii
0.025
-
cellobiose electron acceptor 1,4-benzoquinone Athelia rolfsii
0.031
-
cellobiose electron acceptor meldola blue Athelia rolfsii
0.053
-
cellobiose electron acceptor 3,5-di-tert-butyl-1,2-benzoquinone Athelia rolfsii
0.12
-
cellobiose
-
Athelia rolfsii
0.49
-
cellotriose
-
Athelia rolfsii
0.54
-
cellopentaose
-
Athelia rolfsii
0.55
-
cellobiose electron acceptor 2,6-dimethyl-1,4-benzoquinone Athelia rolfsii
0.6
-
cellotetraose
-
Athelia rolfsii
2
-
beta-lactose 70-75% Athelia rolfsii
2.3
-
thiocellobiose
-
Athelia rolfsii
2.4
-
lactose
-
Athelia rolfsii
5.4
-
xylobiose
-
Athelia rolfsii
54
-
alpha-lactose
-
Athelia rolfsii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Athelia rolfsii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
101000
-
MALDI-MS Athelia rolfsii
110000
-
1 * 110000, SDS-PAGE Athelia rolfsii
126000
-
native PAGE Athelia rolfsii

Organism

Organism UniProt Comment Textmining
Athelia rolfsii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE sepharose, Resource PHE Athelia rolfsii

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Athelia rolfsii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
63
-
electron acceptor 2,6-dichloroindophenol Athelia rolfsii
66
-
electron acceptor cytochrome c Athelia rolfsii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-lactose + acceptor
-
Athelia rolfsii 4-O-(alpha-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
?
beta-lactose + acceptor
-
Athelia rolfsii 4-O-(beta-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
?
cellobiose + acceptor artificial electron acceptors: 2,6-dichlorophenolindophenol, 1,4-benzoquinone, 2,6-dimethyl-1,4-benzoquinone, 2,6-dimethoxy-1,4-benzoquinone, 3,5-di-tert-butyl-1,2-benzoquinone, methylene blue and green, meldola blue, 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid), cytochrome c, K3Fe(CN)6 Athelia rolfsii cellobiono-1,5-lactone + reduced acceptor
-
?
cellopentaose + acceptor
-
Athelia rolfsii cellopentaono-1,5-lactone + reduced acceptor
-
?
cellotetraose + acceptor
-
Athelia rolfsii cellotetrono-1,5-lactone + reduced acceptor
-
?
cellotriose + acceptor
-
Athelia rolfsii cellotriono-1,5-lactone + reduced acceptor
-
?
lactose + acceptor
-
Athelia rolfsii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
?
thiocellobiose + acceptor
-
Athelia rolfsii thiocellobiono-1,5-lactone + reduced acceptor
-
?
xylobiose + 2,6-dichlorophenolindophenol
-
Athelia rolfsii 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
r

Subunits

Subunits Comment Organism
monomer 1 * 110000, SDS-PAGE Athelia rolfsii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
cellobiose electron acceptor 2,6-dimethoxy-1,4-benzoquinone Athelia rolfsii
15
-
cellobiose electron acceptor 2,6-dimethyl-1,4-benzoquinone Athelia rolfsii
17
-
alpha-lactose
-
Athelia rolfsii
18
-
cellobiose electron acceptors methylene blue or methylene green Athelia rolfsii
23
-
cellobiose electron acceptor 3,5-di-tert-butyl-1,2-benzoquinone Athelia rolfsii
24
-
cellotetraose
-
Athelia rolfsii
24
-
cellopentaose
-
Athelia rolfsii
24
-
cellobiose electron acceptor meldola blue Athelia rolfsii
26
-
lactose
-
Athelia rolfsii
26
-
cellotriose
-
Athelia rolfsii
27
-
cellobiose
-
Athelia rolfsii
27
-
xylobiose
-
Athelia rolfsii
27
-
thiocellobiose
-
Athelia rolfsii
27
-
cellobiose electron acceptor 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid) cation radical Athelia rolfsii
30
-
cellobiose 2,6-dichloroindophenpl or 1,4-benzoquinone as electron acceptor Athelia rolfsii
34
-
cellobiose electron acceptor cytochrome c from horseheart Athelia rolfsii
37
-
cellobiose electron acceptor K3Fe(CN)6 Athelia rolfsii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7 5 depends on electron acceptor Athelia rolfsii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.7
-
at 35°C, enzyme loses 50% of its initial activity after 10 min at pH 2.2, stability optimum shifts to 5.0 at 46°C with a half-life of 12 h Athelia rolfsii

Cofactor

Cofactor Comment Organism Structure
FAD 1 heme per enzyme molecule, 1 FAD nucleotide per molecule Athelia rolfsii
heme b 1 heme per enzyme molecule, 1 FAD nucleotide per molecule Athelia rolfsii
additional information limited proteolytic cleavage of the enzyme leads to an inactive heme peptide and an active FAD domain which has been termed cellobiose: quinone 1-oxidoreductase EC 1.5.1.1 Athelia rolfsii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
31
-
lactobiono-1,5-lactone
-
Athelia rolfsii