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Literature summary for 1.1.99.18 extracted from
- Cloning and characterization of a thermostable cellobiose dehydrogenase from Sporotrichum thermophile (1999), Arch. Biochem. Biophys., 365, 223-230.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermothelomyces heterothallicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00067 | - |
cytochrome c | at 25°C | Thermothelomyces heterothallicus |  |
| 0.00067 | - |
cytochrome c | at pH 4.5 and 25°C | Thermothelomyces heterothallicus | |
| 0.001 | - |
cytochrome c | at 45°C | Thermothelomyces heterothallicus | |
| 0.001 | - |
cytochrome c | at pH 4.5 and 45°C | Thermothelomyces heterothallicus | |
| 0.0024 | - |
2,6-dichlorophenolindophenol | at 25°C | Thermothelomyces heterothallicus | |
| 0.0024 | - |
2,6-dichlorophenolindophenol | at pH 4.5 and 25°C | Thermothelomyces heterothallicus | |
| 0.0034 | - |
cellobiose | at 25°C | Thermothelomyces heterothallicus | |
| 0.0037 | - |
2,6-dichlorophenolindophenol | at 45°C | Thermothelomyces heterothallicus | |
| 0.0037 | - |
2,6-dichlorophenolindophenol | at pH 4.5 and 45°C | Thermothelomyces heterothallicus | |
| 0.0045 | - |
ferricyanide | at 25°C | Thermothelomyces heterothallicus | |
| 0.0045 | - |
ferricyanide | at pH 4.5 and 25°C | Thermothelomyces heterothallicus | |
| 0.0058 | - |
cellobiose | at 25°C | Thermothelomyces heterothallicus | |
| 0.0064 | - |
cellotetraose | at 25°C | Thermothelomyces heterothallicus | |
| 0.0067 | - |
ferricyanide | at 45°C | Thermothelomyces heterothallicus | |
| 0.0067 | - |
ferricyanide | at pH 4.5 and 45°C | Thermothelomyces heterothallicus | |
| 0.011 | - |
cellotetraose | at 25°C | Thermothelomyces heterothallicus | |
| 0.0281 | - |
lactose | at 25°C | Thermothelomyces heterothallicus | |
| 0.05 | - |
lactose | at 45°C | Thermothelomyces heterothallicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Phanerodontia chrysosporium | - |
- |
| extracellular | - |
Thermothelomyces heterothallicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
gel filtration | Thermothelomyces heterothallicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | - |
- |
- |
| Thermothelomyces heterothallicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Phanerodontia chrysosporium |
| glycoprotein | difference in apparent molecular weight and that observed by SDS-PAGE might be due to glycosylation, six potential glycosylation sites in enzyme amino acid sequence | Thermothelomyces heterothallicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Phenyl-Sepharose, Sephacryl S-200, Mono Q | Thermothelomyces heterothallicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Phanerodontia chrysosporium | - |
| culture medium | - |
Thermothelomyces heterothallicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
at pH 4.0 and 25°C | Thermothelomyces heterothallicus |
| 11.5 | - |
at 40°C | Phanerodontia chrysosporium |
| 13.4 | - |
cytochrome c reduction at 60°C | Thermothelomyces heterothallicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + 2,6-dichlorophenolindophenol | - |
Thermothelomyces heterothallicus | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
r | |
| cellobiose + acceptor | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
| cellobiose + acceptor | electron acceptors: phenol blue, Fe3+, methylene blue, 3,5-di-tert-butyl-1,2-benzoquinone, p-benzoquinone, potassium ferricyanide, cytochrome c | Thermothelomyces heterothallicus | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
| cellobiose + cytochrome c | - |
Thermothelomyces heterothallicus | cellobiono-1,5-lactone + reduced cytochrome c | - |
r | |
| cellobiose + ferricyanide | - |
Thermothelomyces heterothallicus | cellobiono-1,5-lactone + ferrocyanide | - |
r | |
| cellotetraose + acceptor | - |
Phanerodontia chrysosporium | cellotetrono-1,5-lactone + reduced acceptor | - |
? | |
| cellotetraose + acceptor | - |
Thermothelomyces heterothallicus | cellotetrono-1,5-lactone + reduced acceptor | - |
? | |
| lactose + acceptor | - |
Phanerodontia chrysosporium | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
| lactose + acceptor | - |
Thermothelomyces heterothallicus | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 95000, SDS-PAGE | Thermothelomyces heterothallicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Phanerodontia chrysosporium |
| 60 | - |
- |
Thermothelomyces heterothallicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | thermostable enzyme, 10 h half-life at 60°C, 3 h at 70°C | Thermothelomyces heterothallicus |
| 60 | - |
25 min half-life at 60°C, instantaneousley inactivated at 70°C | Phanerodontia chrysosporium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.9 | - |
lactose | at 25°C | Thermothelomyces heterothallicus | |
| 6.1 | - |
cellobiose | at 25°C | Thermothelomyces heterothallicus | |
| 6.1 | - |
cellotetraose | at 25°C | Thermothelomyces heterothallicus | |
| 7.3 | - |
cytochrome c | at 25°C | Thermothelomyces heterothallicus | |
| 7.5 | - |
2,6-dichlorophenolindophenol | at 25°C | Thermothelomyces heterothallicus | |
| 12.8 | - |
ferricyanide | at 25°C | Thermothelomyces heterothallicus | |
| 15.1 | - |
cellotetraose | at 25°C | Thermothelomyces heterothallicus | |
| 16.1 | - |
cellobiose | at 25°C | Thermothelomyces heterothallicus | |
| 16.8 | - |
lactose | at 45°C | Thermothelomyces heterothallicus | |
| 18.3 | - |
2,6-dichlorophenolindophenol | at 45°C | Thermothelomyces heterothallicus | |
| 20.8 | - |
cytochrome c | at 45°C | Thermothelomyces heterothallicus | |
| 24.1 | - |
ferricyanide | at 45°C | Thermothelomyces heterothallicus | |
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Release 2023.1 (January 2023)
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