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Literature summary for 1.1.99.18 extracted from

  • Fang, J.; Liu, W.; Gao, P.J.
    Cellobiose dehydrogenase from Schizophyllum commune: purification and study of some catalytic, inactivation, and cellulose-binding properties (1998), Arch. Biochem. Biophys., 353, 37-46.
    View publication on PubMed
Search for more literature for 1.1.99.18

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 2 mM, 70% inhibition after 6 h Schizophyllum commune
Hg2+ 2 mM, 20% inhibition after 6 h Schizophyllum commune
SDS 5 mM, complete inhibition Schizophyllum commune
Zn2+ 2 mM, 60% inhibition after 6 h Schizophyllum commune

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 cytochrome c
-
 Schizophyllum commune
0.01
-
 2,6-dichlorphenolindophenol
-
 Schizophyllum commune
0.03
-
 cellobiose
-
 Schizophyllum commune
0.058
-
 cellotriose
-
 Schizophyllum commune
0.06
-
 cellotetraose
-
 Schizophyllum commune
0.071
-
 ferricyanide
-
 Schizophyllum commune
0.085
-
 cellopentaose
-
 Schizophyllum commune
0.108
-
 lactose
-
 Schizophyllum commune

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Schizophyllum commune
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
102000
-
 x * 102000, SDS-PAGE Schizophyllum commune

Organism

Organism UniProt Comment Textmining
Schizophyllum commune
-
 white-rot fungus
-
Schizophyllum commune AS 5.391
-
 white-rot fungus
-

Oxidation Stability

Oxidation Stability Organism
very stable against H2O2 inactivation, 25 mM H2O2, pH 5.0, no loss in activity after 24 h, at 50 mM H2O2 27% enzyme activity are lost within 12 h, at 1 mM H2O2 with addition of 2 mM cellobiose and 0.05 mM FeCl3 all enzyme activity is lost within 4 h Schizophyllum commune

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 14% carbohydrate content Schizophyllum commune

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Sephadex A50, Sephadex G-100 Schizophyllum commune

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Schizophyllum commune
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.4
-
-
 Schizophyllum commune

Storage Stability

Storage Stability Organism
4°C, several months Schizophyllum commune

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenolindophenol
-
 Schizophyllum commune cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 r
cellobiose + acceptor electron acceptors: cytochrome c, 2,6-dichlorphenolindophenol, ferricyanide. O2 is reduced to H2O2 Schizophyllum commune cellobiono-1,5-lactone + reduced acceptor
-
 ?
cellobiose + acceptor electron acceptors: cytochrome c, 2,6-dichlorphenolindophenol, ferricyanide. O2 is reduced to H2O2 Schizophyllum commune AS 5.391 cellobiono-1,5-lactone + reduced acceptor
-
 ?
cellobiose + cytochrome c
-
 Schizophyllum commune cellobiono-1,5-lactone + reduced cytochrome c
-
 r
cellobiose + ferricyanide
-
 Schizophyllum commune cellobiono-1,5-lactone + ferrocyanide
-
 r
cellopentaose + acceptor
-
 Schizophyllum commune cellopentaono-1,5-lactone + reduced acceptor
-
 ?
cellopentaose + acceptor
-
 Schizophyllum commune AS 5.391 cellopentaono-1,5-lactone + reduced acceptor
-
 ?
cellotetraose + acceptor
-
 Schizophyllum commune cellotetrono-1,5-lactone + reduced acceptor
-
 ?
cellotetraose + acceptor
-
 Schizophyllum commune AS 5.391 cellotetrono-1,5-lactone + reduced acceptor
-
 ?
cellotriose + acceptor
-
 Schizophyllum commune cellotriono-1,5-lactone + reduced acceptor
-
 ?
cellotriose + acceptor
-
 Schizophyllum commune AS 5.391 cellotriono-1,5-lactone + reduced acceptor
-
 ?
lactose + acceptor
-
 Schizophyllum commune 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
 ?
lactose + acceptor
-
 Schizophyllum commune AS 5.391 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
? x * 102000, SDS-PAGE Schizophyllum commune

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 loss of 40% activity after 8 h at 45°C, enzyme is almost completely inactivated after 4 h at 50° Schizophyllum commune

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
 lactose
-
 Schizophyllum commune
10
-
 cellopentaose
-
 Schizophyllum commune
12
-
 cellotriose
-
 Schizophyllum commune
12
-
 cellotetraose
-
 Schizophyllum commune
13
-
 cellobiose
-
 Schizophyllum commune
13.5
-
 ferricyanide
-
 Schizophyllum commune
25
-
 cytochrome c
-
 Schizophyllum commune
63
-
 2,6-dichlorphenolindophenol
-
 Schizophyllum commune

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Schizophyllum commune

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 at 30°C stable for 24 h, at pH 3.5 almost 40% activity are lost within 12 h Schizophyllum commune

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Schizophyllum commune
heme b
-
 Schizophyllum commune