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Literature summary for 1.1.99.18 extracted from
- Cellobiose dehydrogenase produced by Monilia sp. (1988), Methods Enzymol., 160, 454-463.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
cellobiose | - |
Monilia sitophila |  |
| 0.08 | - |
2,6-dichlorophenolindophenol | - |
Monilia sitophila | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | bound to mycelium from which it is released into the extracellular medium | Monilia sitophila | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
gel filtration | Monilia sitophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Monilia sitophila | - |
inducible enzyme | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | bound to mycelium from which it is released into the extracellular medium | Monilia sitophila | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
27.2 micromol/min*ml enzyme, electron acceptor 2,6-dichlorophenolindophenol, enzyme activity in crude extracts of seven day old culture | Monilia sitophila |
| additional information | - |
15.6 micromol/min*ml enzyme, electron acceptor phenol blue, enzyme activity in crude extracts of seven day old culture | Monilia sitophila |
| additional information | - |
6.4 micromol/min ml enzyme, electron acceptor cytochrome c, enzyme activity in crude extracts of seven day old culture | Monilia sitophila |
| additional information | - |
4.9 micromol/min ml enzyme, electron acceptor potassium ferricyanide, enzyme activity in crude extracts of seven day old culture | Monilia sitophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-beta-glucosylmannose + acceptor | 47% the rate of cellobiose oxidation | Monilia sitophila | ? | - |
? | |
| 4-methylumbelliferyl-beta-D-cellobioside + acceptor | 70% the rate of cellobiose oxidation | Monilia sitophila | ? | - |
? | |
| cello-oligosaccharides + acceptor | 67% the rate of cellobiose oxidation | Monilia sitophila | ? | - |
? | |
| cellobiose + 2,6-dichlorophenolindophenol | - |
Monilia sitophila | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
r | |
| cellobiose + acceptor | electron acceptors: 2,6-dichlorophenolindophenol, cytochrome c, phenol blue, potassium ferricyanide at a low rate | Monilia sitophila | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
| lactose + acceptor | 60% the rate of cellobiose oxidation | Monilia sitophila | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 4.5 | - |
Monilia sitophila |
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Release 2023.1 (January 2023)
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