Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.99.14 extracted from

  • Eisenhut, M.; Kahlon, S.; Hasse, D.; Ewald, R.; Lieman-Hurwitz, J.; Ogawa, T.; Ruth, W.; Bauwe, H.; Kaplan, A.; Hagemann, M.
    The plant-like C2 glycolate cycle and the bacterial-like glycerate pathway cooperate in phosphoglycolate metabolism in cyanobacteria (2006), Plant Physiol., 142, 333-342.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.14

Cloned(Commentary)

Cloned (Comment) Organism
gene glcD, expression of N-terminally Strep-tagged enzyme in Escherichia coli strain BL21 Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
additional information construction of GlcD-deficient mutants, co-mutation of other enzymes of the alternative bacterial glycerate pathway, progressive segregation, genotyping and metabolic analysis, growth phenotypes under normal and carbon limited conditions, overview Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycolate + NAD+ Synechocystis sp. the enzyme is involved in the bacterial 2-phosphoglycolate pathway, overview glyoxylate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
 strain PCC 6803, gene glcD
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally Strep-tagged enzyme from Escherichia coli strain BL21 by streptavidin-tactin affinity chromatography Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 determination of internal glycolate concentrations Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycolate + NAD+
-
 Synechocystis sp. glyoxylate + NADH
-
 ?
glycolate + NAD+ the enzyme is involved in the bacterial 2-phosphoglycolate pathway, overview Synechocystis sp. glyoxylate + NADH
-
 ?

Synonyms

Synonyms Comment Organism
GlcD
-
 Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Synechocystis sp.