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Literature summary for 1.1.99.14 extracted from
- Glycolate oxidoreductase in Escherichia coli (1971), Biochim. Biophys. Acta, 267, 227-237.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | - |
Escherichia coli |  |
| cytochrome c | inhibits rate of phenazine methosulfate stimulated dichlorophenolindophenol reduction | Escherichia coli | |
| HgCl2 | complete | Escherichia coli | |
| K3[Fe(CN)6] | complete inhibition | Escherichia coli | |
| KCN | - |
Escherichia coli | |
| additional information | high ionic strength; overview over inhibitors at different concentrations | Escherichia coli | |
| PCMB | - |
Escherichia coli | |
| ZnCl2 | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
glycolate | - |
Escherichia coli | |
| 0.7 | - |
D(-)-lactate | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Escherichia coli | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycolate + acceptor | Escherichia coli | the bacterial enzyme catalyzes phenazine methosulfate dependent reduction of 2,6-dichlorophenolindophenol or cytochrome c | glyocylate + reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially, from cells grown on glycolate | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.14 | - |
glyoxylate formation in the presence of phenazine methosulfate | Escherichia coli |
| 0.15 | - |
crude extract | Escherichia coli |
| 3.1 | - |
partially purified protein | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 2°C, half-life of 10 days, FMN or FAD do not restore activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + acceptor | best substrate | Escherichia coli | pyruvate + reduced acceptor | - |
? | |
| (R)-lactate + acceptor | artificial acceptor 2,6-dichlorophenolindophenol is reduced via phenazine methosulfate, cytochrome c can replace 2,6-dichlorophenolindophenol | Escherichia coli | pyruvate + reduced acceptor | - |
? | |
| (S)-lactate + acceptor | about 16% activity of glycolate | Escherichia coli | pyruvate + reduced acceptor | - |
? | |
| DL-malate + acceptor | about 3% activity of glycolate | Escherichia coli | 2-oxosuccinate + reduced acceptor | - |
? | |
| glycolate + acceptor | artificial acceptor 2,6-dichlorophenolindophenol is reduced via phenazine methosulfate, cytochrome c can replace 2,6-dichlorophenolindophenol | Escherichia coli | glyoxylate + reduced acceptor | - |
? | |
| glycolate + acceptor | the bacterial enzyme catalyzes phenazine methosulfate dependent reduction of 2,6-dichlorophenolindophenol or cytochrome c | Escherichia coli | glyocylate + reduced acceptor | - |
? | |
| glyoxylate + acceptor | about 6% activity of glycolate | Escherichia coli | oxalate + reduced acceptor | - |
? | |
| phosphoglycolate + acceptor | about 9% activity of glycolate | Escherichia coli | ? + reduced acceptor | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.8 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 9 | about half-maximal activity at pH 7.3 and 9.0 | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | in vitro requirement, terminal acceptor | Escherichia coli | |
| cytochrome c | can replace dichlorophenolindophenol as acceptor | Escherichia coli | |
| additional information | FAD, FMN, NAD+, NADP+, oxidized glutathione, KNO3, ferricyanide, FeCl3, cytochrome c do not stimulate electron transfer from glycolate to dichlorophenolindophenol | Escherichia coli | |
| phenazine methosulfate | transfers electrons from the substrate preferentially to 2,6-dichlorophenolindophenol | Escherichia coli | |
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