Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 1 mM causes 100% inhibition | Pseudomonas aeruginosa | |
Benzyltrimethylammonium chloride | slight | Pseudomonas aeruginosa | |
Betaine hydrochloride | slight | Pseudomonas aeruginosa | |
CuSO4 | 1 mM causes 100% inhibition | Pseudomonas aeruginosa | |
dimethylglycine | slight | Pseudomonas aeruginosa | |
DL-Carnitine hydrochloride | slight | Pseudomonas aeruginosa | |
glycine | slight | Pseudomonas aeruginosa | |
HgCl2 | 1 mM causes 100% inhibition | Pseudomonas aeruginosa | |
iodoacetic acid | - |
Pseudomonas aeruginosa | |
KCN | 1 mM inhibits | Pseudomonas aeruginosa | |
Mercuric acetate | - |
Pseudomonas aeruginosa | |
MnCl2 | 1 mM causes 100% inhibition | Pseudomonas aeruginosa | |
p-chloromercuribenzoate | - |
Pseudomonas aeruginosa | |
Phenyltriethylammonium iodide | slight | Pseudomonas aeruginosa | |
Tetra-n-butylammonium iodide | slight | Pseudomonas aeruginosa | |
Tetra-n-propylammonium iodide | slight | Pseudomonas aeruginosa | |
Tetraethylammonium iodide | slight | Pseudomonas aeruginosa | |
Tetramethylammonium iodide | slight | Pseudomonas aeruginosa | |
Triethylbenzylammonium iodide | slight | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
phenazine methosulfate | - |
Pseudomonas aeruginosa | |
1.7 | - |
choline | - |
Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
particle-bound | - |
Pseudomonas aeruginosa | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
A-16 strain | - |
Pseudomonas aeruginosa A-16 | - |
A-16 strain | - |
Purification (Comment) | Organism |
---|---|
partial, using Triton-treatment, chloroform-treatment and column chromatography on Sepharose 6B | Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-20°C , stable | Pseudomonas aeruginosa |
5°C, pH 7.4, complete loss of activity, partially purified enzyme, 5-6 days | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + acceptor | acceptor 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazolium bromide, particulate enzyme: 12.4% relative activity to phenazine methosulfate | Pseudomonas aeruginosa | betaine aldehyde + reduced acceptor | - |
? | |
choline + acceptor | cytochrome c, particulate enzyme: 3.1% relative activity to phenazine methosulfate | Pseudomonas aeruginosa | betaine aldehyde + reduced acceptor | - |
? | |
choline + acceptor | acceptor ferricyanide, particulate enzyme: 112% relative activity to phenazine methosulfate | Pseudomonas aeruginosa | betaine aldehyde + reduced acceptor | - |
? | |
choline + acceptor | acceptor 2,6-dichlorophenolindophenol, particulate enzyme: 3.2% relative activity to phenazine methosulfate | Pseudomonas aeruginosa | betaine aldehyde + reduced acceptor | - |
? | |
choline + acceptor | acceptor: phenazine methosulfate, 100% relative activity | Pseudomonas aeruginosa | betaine aldehyde + reduced acceptor | - |
? | |
choline + acceptor | electron acceptor: phenazine methosulfate | Pseudomonas aeruginosa | betaine aldehyde + reduced acceptor | - |
? | |
choline + acceptor | acceptor 3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyltetrazolium bromide, particulate enzyme: 12.4% relative activity to phenazine methosulfate | Pseudomonas aeruginosa A-16 | betaine aldehyde + reduced acceptor | - |
? | |
choline + acceptor | cytochrome c, particulate enzyme: 3.1% relative activity to phenazine methosulfate | Pseudomonas aeruginosa A-16 | betaine aldehyde + reduced acceptor | - |
? | |
choline + acceptor | acceptor ferricyanide, particulate enzyme: 112% relative activity to phenazine methosulfate | Pseudomonas aeruginosa A-16 | betaine aldehyde + reduced acceptor | - |
? | |
choline + acceptor | acceptor 2,6-dichlorophenolindophenol, particulate enzyme: 3.2% relative activity to phenazine methosulfate | Pseudomonas aeruginosa A-16 | betaine aldehyde + reduced acceptor | - |
? | |
choline + acceptor | acceptor: phenazine methosulfate, 100% relative activity | Pseudomonas aeruginosa A-16 | betaine aldehyde + reduced acceptor | - |
? | |
choline + phenazine methosulfate | - |
Pseudomonas aeruginosa | betaine aldehyde + reduced phenazine methosulfate | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
10 min, pH 8.3, 10% loss of activity | Pseudomonas aeruginosa |
40 | - |
10 min, pH 8.3, 50% loss of activity | Pseudomonas aeruginosa |
50 | - |
10 min, pH 8.3, 100% loss of activity | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | about 50% of activity maximum at pH 7.5 and pH 10.0 | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme requires no readily dissociable coenzyme for activity | Pseudomonas aeruginosa |