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Literature summary for 1.1.99.1 extracted from
- Studies on choline dehydrogenase (1959), J. Biol. Chem., 234, 1605-1610.
General Stability
| General Stability | Organism |
|---|---|
| 25 cycles of freezing/thawing cause 40% loss of activity | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7 | - |
choline | electron acceptor: phenazine methosulfate | Rattus norvegicus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, pH 7.4 as mitochondrial acetone powder, 20% loss of activity after 6 days | Rattus norvegicus |
| -15°C, pH 7.4 in extracts, 13% loss of activity after 6 days | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline + acceptor | acceptor O2: 73% relative activity to phenazine methosulfate | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | acceptor 2,6-dichlorophenolindophenol: 27% relative activity to phenazine methosulfate | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | cytochrome c, ferricyanide, methylene blue and 2,6-dichlorophenolindophenol show no direct reaction with the enzyme | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | acceptor methylene blue: 4% relative activity to phenazine methosulfate | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | acceptor cytochrome c: 63% relative activity to phenazine methosulfate | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | acceptor: phenazine methosulfate, 100% relative activity | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | electron acceptor: phenazine methosulfate | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
? | |
| choline + acceptor | acceptor ferricyanide: 42% relative activity to phenazine methosulfate | Rattus norvegicus | betaine aldehyde + reduced acceptor | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
3 min, pH 7, 63% inactivation | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 8.2 | crude enzyme extract | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme requires no readily dissociable coenzyme for activity | Rattus norvegicus |
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Release 2023.1 (January 2023)
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