Literature summary for 1.1.98.5 extracted from

Bleicher, K.; Winter, J.
Purification and properties of F420- and NADP(+)-dependent alcohol dehydrogenases of Methanogenium liminatans and Methanobacterium palustre, specific for secondary alcohols (1991), Eur. J. Biochem., 200, 43-51.

Search for more literature for 1.1.98.5

General Stability

General Stability	Organism
unstable in 40 mM phosphate or Tris-HC1 buffer at 4°C and also in low-ionic-strength buffers in which precipitation occurrs and enzyme activity is lost irreversibly. The enzyme can be stabilized by addition of 200 mM sodium sulfate and 10 mM 2-propanol to the buffer	Methanofollis liminatans

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	5 mM, 87% inhibition	Methanofollis liminatans
2,2'-dipyridyl	2 mM, 18% inhibition	Methanofollis liminatans
4-hydroxymercuribenzoate	0.1 mM, complete inhibition	Methanofollis liminatans
KCl	-	Methanofollis liminatans
additional information	no inhibition be EDTA (2-5 mM), iodoacetate (2-5 mM), or 4-hydroxymercurobenzoate (0.01-0.1 mM)	Methanofollis liminatans
NaCl	-	Methanofollis liminatans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.008	-	reduced coenzyme F420	pH 5.8, 37°C	Methanofollis liminatans
0.018	-	oxidized coenzyme F420	pH 5.8, 37°C	Methanofollis liminatans
0.5	-	propanone	pH 5.8, 37°C	Methanofollis liminatans
0.8	-	(S)-2-butanol	pH 5.8, 37°C	Methanofollis liminatans
1.2	-	2-butanol	pH 5.8, 37°C	Methanofollis liminatans
2.01	-	(R)-2-butanol	pH 5.8, 37°C	Methanofollis liminatans
2.2	-	2-propanol	pH 5.8, 37°C	Methanofollis liminatans
4.3	-	Cyclopentanone	pH 5.8, 37°C	Methanofollis liminatans
6.1	-	Cyclopentanol	pH 5.8, 37°C	Methanofollis liminatans
7.2	-	2-Pentanol	pH 5.8, 37°C	Methanofollis liminatans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	the enzyme contains no zinc ions	Methanofollis liminatans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39000	-	4 * 39000, SDS-PAGE	Methanofollis liminatans
150000	-	gel filtration	Methanofollis liminatans

Organism

Organism	UniProt	Comment	Textmining
Methanofollis liminatans	-	-	-
Methanofollis liminatans DSM 4140	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Methanofollis liminatans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-2-butanol + oxidized coenzyme F420	-	Methanofollis liminatans	2-butanone + reduced coenzyme F420	-	r
(R)-2-butanol + oxidized coenzyme F420	-	Methanofollis liminatans DSM 4140	2-butanone + reduced coenzyme F420	-	r
(S)-2-butanol + oxidized coenzyme F420	-	Methanofollis liminatans	2-butanone + reduced coenzyme F420	-	r
(S)-2-butanol + oxidized coenzyme F420	-	Methanofollis liminatans DSM 4140	2-butanone + reduced coenzyme F420	-	r
2-butanone + reduced coenzyme F420	-	Methanofollis liminatans	2-butanol + oxidized coenzyme F420	-	r
2-butanone + reduced coenzyme F420	-	Methanofollis liminatans DSM 4140	2-butanol + oxidized coenzyme F420	-	r
2-pentanol + oxidized coenzyme F420	-	Methanofollis liminatans	2-pentanone + reduced coenzyme F420	-	r
2-pentanol + oxidized coenzyme F420	-	Methanofollis liminatans DSM 4140	2-pentanone + reduced coenzyme F420	-	r
2-pentanone + reduced coenzyme F420	-	Methanofollis liminatans	2-pentanol + oxidized coenzyme F420	-	r
2-propanol + oxidized coenzyme F420	-	Methanofollis liminatans	2-propanone + reduced coenzyme F420	2-propanone i.e. acetone	r
cyclopentanol + oxidized coenzyme F420	-	Methanofollis liminatans	cyclopentanone + reduced coenzyme F420	-	r
cyclopentanone + reduced coenzyme F420	-	Methanofollis liminatans	cyclopentanol + oxidized coenzyme F420	-	r
additional information	the enzyme catalysed the oxidation of various secondary and cyclic alcohols to the corresponding ketones and the reverse reaction. No primary alcohols are oxidized. 1,3-butandiol, glycerol, methanol, ethanol, 1,2-ethandiol, 1 -propanol, 1-amino-2-propanol, 1-butanol, 1-pentanol, 1-hexanol and benzylalcohol are not oxidized nor are the respective aldehydes or ketones reduced	Methanofollis liminatans	?	-	?
additional information	the enzyme catalysed the oxidation of various secondary and cyclic alcohols to the corresponding ketones and the reverse reaction. No primary alcohols are oxidized. 1,3-butandiol, glycerol, methanol, ethanol, 1,2-ethandiol, 1 -propanol, 1-amino-2-propanol, 1-butanol, 1-pentanol, 1-hexanol and benzylalcohol are not oxidized nor are the respective aldehydes or ketones reduced	Methanofollis liminatans DSM 4140	?	-	?
propanone + reduced coenzyme F420	2-propanone i.e. acetone	Methanofollis liminatans	2-propanol + oxidized coenzyme F420	-	r

Subunits

Subunits	Comment	Organism
tetramer	4 * 39000, SDS-PAGE	Methanofollis liminatans

Synonyms

Synonyms	Comment	Organism
F420-dependent alcohol dehydrogenase	-	Methanofollis liminatans
secondary alcohol:F420 oxidoreductase	-	Methanofollis liminatans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
42	-	-	Methanofollis liminatans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.3	-	2-Pentanone	pH 5.8, 37°C	Methanofollis liminatans
2.1	-	2-butanone	pH 5.8, 37°C	Methanofollis liminatans
4.4	-	Cyclopentanone	pH 5.8, 37°C	Methanofollis liminatans
12.8	-	Cyclopentanol	pH 5.8, 37°C	Methanofollis liminatans
23.1	-	2-Pentanol	pH 5.8, 37°C	Methanofollis liminatans
38.5	-	(S)-2-butanol	pH 5.8, 37°C	Methanofollis liminatans
49	-	2-butanol	pH 5.8, 37°C	Methanofollis liminatans
51.3	-	(R)-2-butanol	pH 5.8, 37°C	Methanofollis liminatans
85	-	2-propanol	pH 5.8, 37°C	Methanofollis liminatans
85	-	oxidized coenzyme F420	pH 5.8, 37°C	Methanofollis liminatans
161.5	-	reduced coenzyme F420	pH 5.8, 37°C	Methanofollis liminatans
161.5	-	propanone	pH 5.8, 37°C	Methanofollis liminatans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Methanofollis liminatans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1	-	Cyclopentanone	pH 5.8, 37°C	Methanofollis liminatans
2.1	-	Cyclopentanol	pH 5.8, 37°C	Methanofollis liminatans
3.2	-	2-Pentanol	pH 5.8, 37°C	Methanofollis liminatans
26	-	(R)-2-butanol	pH 5.8, 37°C	Methanofollis liminatans
38.6	-	2-propanol	pH 5.8, 37°C	Methanofollis liminatans
40.8	-	2-butanol	pH 5.8, 37°C	Methanofollis liminatans
48	-	(S)-2-butanol	pH 5.8, 37°C	Methanofollis liminatans
323	-	2-Pentanone	pH 5.8, 37°C	Methanofollis liminatans
4700	-	oxidized coenzyme F420	pH 5.8, 37°C	Methanofollis liminatans
20200	-	reduced coenzyme F420	pH 5.8, 37°C	Methanofollis liminatans

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Release 2023.1 (January 2023)