Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Pichia pastoris | Rasamsonia emersonii |
expression in Escherichia coli and Pichia pastoris | Thermoascus crustaceus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
D-glucose | pH 7.0, 37°C, glycosylated recombinant protein | Thermoascus crustaceus | |
4.6 | - |
D-glucose | pH 7.0, 37°C, unglycosylated recombinant protein | Thermoascus crustaceus | |
337 | - |
D-glucose | pH 7.0, 37°C, glycosylated recombinant protein | Rasamsonia emersonii | |
406 | - |
D-glucose | pH 7.0, 37°C, unglycosylated recombinant protein | Rasamsonia emersonii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | signal peptide consists of residues Met1 to Ala16 | Thermoascus crustaceus | - |
- |
extracellular | signal peptide consists of residues Met1 to Ala17 | Rasamsonia emersonii | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rasamsonia emersonii | A0A1E1GL61 | - |
- |
Thermoascus crustaceus | A0A1E1GL87 | - |
- |
Thermoascus crustaceus NBRC 9129 | A0A1E1GL87 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | x * 80000-200000, recombinant protein produced in Pichia pastoris, SDS-PAGE. x * 58000, unglycosylated protein | Thermoascus crustaceus |
glycoprotein | x * 90000-200000, recombinant protein produced in Pichia pastoris, SDS-PAGE. x * 65000, unglycosylated protein | Rasamsonia emersonii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
pH 7.0, 37°C, specific activity of unglycosylated recombinant protein is 16000 U/microM, specific activity of glycosylated recombinant protein is 13500 U/microM | Thermoascus crustaceus |
additional information | - |
pH 7.0, 37°C, specific activity of unglycosylated recombinant protein is 9210 U/microM, specific activity of glycosylated recombinant protein is 8790 U/microM | Rasamsonia emersonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + 2,6-dichlorophenolindophenol | - |
Rasamsonia emersonii | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | - |
Thermoascus crustaceus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | - |
Thermoascus crustaceus NBRC 9129 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
additional information | no substrate: maltose | Rasamsonia emersonii | ? | - |
? | |
additional information | no substrate: maltose | Thermoascus crustaceus | ? | - |
? | |
additional information | no substrate: maltose | Thermoascus crustaceus NBRC 9129 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000-200000, recombinant protein produced in Pichia pastoris, SDS-PAGE. x * 58000, unglycosylated protein | Thermoascus crustaceus |
? | x * 90000-200000, recombinant protein produced in Pichia pastoris, SDS-PAGE. x * 65000, unglycosylated protein | Rasamsonia emersonii |
Synonyms | Comment | Organism |
---|---|---|
FADGH | - |
Rasamsonia emersonii |
FADGH | - |
Thermoascus crustaceus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
ungylcosylated recombinant protein | Rasamsonia emersonii |
60 | - |
ungylcosylated recombinant protein | Thermoascus crustaceus |
65 | - |
gylcosylated recombinant protein | Rasamsonia emersonii |
65 | - |
gylcosylated recombinant protein | Thermoascus crustaceus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | ungylcosylated recombinant protein | Thermoascus crustaceus |
55 | 60 | ungylcosylated recombinant protein | Rasamsonia emersonii |
60 | 65 | gylcosylated recombinant protein | Thermoascus crustaceus |
65 | 70 | gylcosylated recombinant protein | Rasamsonia emersonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
15 min, no loss of activity | Rasamsonia emersonii |
45 | - |
15 min, no loss of activity | Thermoascus crustaceus |
51.6 | - |
melting temperature, unglycosylated recombinant protein | Thermoascus crustaceus |
52.7 | - |
melting temperature, unglycosylated recombinant protein | Rasamsonia emersonii |
60 | - |
15 min, unglycosylated recombinant protein, complete loss of activity, glycosylated recombinant protein, 75% residual activity | Thermoascus crustaceus |
60 | - |
15 min, unglycosylated recombinant protein, complete loss of activity, glycosylated recombinant protein, 89% residual activity | Rasamsonia emersonii |
62.5 | - |
melting temperature, glycosylated recombinant protein | Thermoascus crustaceus |
66.4 | - |
melting temperature, glycosylated recombinant protein | Rasamsonia emersonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
both glycosylated and unglycosylated recombinant protein | Thermoascus crustaceus |
5 | - |
glycosylated recombinant protein | Rasamsonia emersonii |
6 | - |
unglycosylated recombinant protein | Rasamsonia emersonii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 9 | glycosylated recombinant protein | Rasamsonia emersonii |
2 | 9 | glycosylated recombinant protein | Thermoascus crustaceus |
3 | 8 | unglycosylated recombinant protein | Rasamsonia emersonii |
3 | 8 | unglycosylated recombinant protein | Thermoascus crustaceus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | purified protein shows major peaks of oxidized flavin at 380 and 450 nm | Rasamsonia emersonii | |
FAD | purified protein shows major peaks of oxidized flavin at 380 and 450 nm | Thermoascus crustaceus |