Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
D-glucose | at pH 6.5, temperature not specified in the publication | Aspergillus niger | |
22 | - |
D-xylose | at pH 6.5, temperature not specified in the publication | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
using 0.05 mM FAD,10% (v/v) glycerol,and 20 mM potassium phosphate buffer, pH 6.5 | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + 2,6-dichlorophenolindophenol | - |
Aspergillus niger | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + 2,6-dichlorophenolindophenol | 1.8% activity compared to D-glucose | Aspergillus niger | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
additional information | less than 1% activity with maltose, cellobiose, lactose, mannose, fructose, allose, galactose, and sucrose | Aspergillus niger | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
FAD glucose dehydrogenase | - |
Aspergillus niger |
GDH | - |
Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Aspergillus niger |