Cloned (Comment) | Organism |
---|---|
gene adh, DNA and amino acid sequence determination and analysis, sequence comparisons | Komagataeibacter europaeus |
gene adh, DNA and amino acid sequence determination and analysis, sequence comparisons | Komagataeibacter intermedius |
gene adhA, DNA and amino acid sequence determination and analysis, sequence comparisons | Acetobacter pasteurianus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Komagataeibacter europaeus | 16020 | - |
membrane | - |
Komagataeibacter intermedius | 16020 | - |
membrane | - |
Acetobacter pasteurianus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
1 * 74000 + 1 * 44000 + 1 * 16000, SDS-PAGE | Acetobacter pasteurianus |
44000 | - |
1 * 74000 + 1 * 44000 + 1 * 16000, SDS-PAGE | Acetobacter pasteurianus |
45000 | - |
1 * 72000 + 1 * 45000, SDS-PAGE | Komagataeibacter europaeus |
45000 | - |
1 * 72000 + 1 * 45000, SDS-PAGE | Komagataeibacter intermedius |
72000 | - |
1 * 72000 + 1 * 45000, SDS-PAGE | Komagataeibacter europaeus |
72000 | - |
1 * 72000 + 1 * 45000, SDS-PAGE | Komagataeibacter intermedius |
74000 | - |
1 * 74000 + 1 * 44000 + 1 * 16000, SDS-PAGE | Acetobacter pasteurianus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Acetobacter pasteurianus | the enzyme activity is correlated with resistance to acetic acid, due to lower enzyme activity in the organism, the growth of Acetobacter pasteurianus on high acetic acid concentrations is limited, overview | ? | - |
? | |
additional information | Komagataeibacter intermedius | the enzyme activity is correlated with resistance to acetic acid, due to lower enzyme activity in the organism, the growth of Gluconacetobacter intermedius on high acetic acid concentrations is limited, overview | ? | - |
? | |
additional information | Komagataeibacter europaeus | high alcohol dehydrogenase activity in the Gluconacetobacter europaeus cells and high acetic acid stability of the purified enzyme represent two of the unique features that enable this species to grow and stay metabolically active at extremely high concentrations of acetic acid | ? | - |
? | |
additional information | Komagataeibacter intermedius JK3 | the enzyme activity is correlated with resistance to acetic acid, due to lower enzyme activity in the organism, the growth of Gluconacetobacter intermedius on high acetic acid concentrations is limited, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter pasteurianus | Q335W4 | AdhA fragment; strains KKP/584 and DSM 3509, gene adhA | - |
Komagataeibacter europaeus | Q44002 | strains VA and DSM 6160, gene adh | - |
Komagataeibacter intermedius | Q335V9 | adh, fragment; gene adh | - |
Komagataeibacter intermedius JK3 | Q335V9 | adh, fragment; gene adh | - |
Purification (Comment) | Organism |
---|---|
native enzyme from strain JK3, by anion exchange and hydroxylapatite chromatography | Komagataeibacter intermedius |
native enzyme from strain KKP/584, by anion exchange and hydroxylapatite chromatography | Acetobacter pasteurianus |
native enzyme from strain V3, by anion exchange and hydroxylapatite chromatography | Komagataeibacter europaeus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | the cells show high enzyme activity | Komagataeibacter europaeus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
179 | - |
purified enzyme | Komagataeibacter europaeus |
192 | - |
purified enzyme | Komagataeibacter intermedius |
205 | - |
purified enzyme | Acetobacter pasteurianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Komagataeibacter europaeus | acetaldehyde + reduced 2,6-dichlorophenol indophenol | - |
? | |
ethanol + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Komagataeibacter intermedius | acetaldehyde + reduced 2,6-dichlorophenol indophenol | - |
? | |
ethanol + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Acetobacter pasteurianus | acetaldehyde + reduced 2,6-dichlorophenol indophenol | - |
? | |
ethanol + 2,6-dichlorophenol indophenol | with phenazine methosulfonate | Komagataeibacter intermedius JK3 | acetaldehyde + reduced 2,6-dichlorophenol indophenol | - |
? | |
additional information | the enzyme activity is correlated with resistance to acetic acid, due to lower enzyme activity in the organism, the growth of Acetobacter pasteurianus on high acetic acid concentrations is limited, overview | Acetobacter pasteurianus | ? | - |
? | |
additional information | the enzyme activity is correlated with resistance to acetic acid, due to lower enzyme activity in the organism, the growth of Gluconacetobacter intermedius on high acetic acid concentrations is limited, overview | Komagataeibacter intermedius | ? | - |
? | |
additional information | high alcohol dehydrogenase activity in the Gluconacetobacter europaeus cells and high acetic acid stability of the purified enzyme represent two of the unique features that enable this species to grow and stay metabolically active at extremely high concentrations of acetic acid | Komagataeibacter europaeus | ? | - |
? | |
additional information | the enzyme activity is correlated with resistance to acetic acid, due to lower enzyme activity in the organism, the growth of Gluconacetobacter intermedius on high acetic acid concentrations is limited, overview | Komagataeibacter intermedius JK3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 72000 + 1 * 45000, SDS-PAGE | Komagataeibacter europaeus |
dimer | 1 * 72000 + 1 * 45000, SDS-PAGE | Komagataeibacter intermedius |
trimer | 1 * 74000 + 1 * 44000 + 1 * 16000, SDS-PAGE | Acetobacter pasteurianus |
Synonyms | Comment | Organism |
---|---|---|
AdhA | - |
Acetobacter pasteurianus |
ADHI | - |
Komagataeibacter europaeus |
PQQ-dependent ADH | - |
Komagataeibacter europaeus |
PQQ-dependent ADH | - |
Komagataeibacter intermedius |
PQQ-dependent ADH | - |
Acetobacter pasteurianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Komagataeibacter europaeus |
7 | - |
assay at | Komagataeibacter intermedius |
7 | - |
assay at | Acetobacter pasteurianus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | - |
Acetobacter pasteurianus | |
pyrroloquinoline quinone | dependent on | Komagataeibacter europaeus | |
pyrroloquinoline quinone | dependent on | Komagataeibacter intermedius |