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Literature summary for 1.1.5.5 extracted from

  • Trcek, J.; Toyama, H.; Czuba, J.; Misiewicz, A.; Matsushita, K.
    Correlation between acetic acid resistance and characteristics of PQQ-dependent ADH in acetic acid bacteria (2006), Appl. Microbiol. Biotechnol., 70, 366-373.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene adh, DNA and amino acid sequence determination and analysis, sequence comparisons Komagataeibacter europaeus
gene adh, DNA and amino acid sequence determination and analysis, sequence comparisons Komagataeibacter intermedius
gene adhA, DNA and amino acid sequence determination and analysis, sequence comparisons Acetobacter pasteurianus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Komagataeibacter europaeus 16020
-
membrane
-
Komagataeibacter intermedius 16020
-
membrane
-
Acetobacter pasteurianus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
1 * 74000 + 1 * 44000 + 1 * 16000, SDS-PAGE Acetobacter pasteurianus
44000
-
1 * 74000 + 1 * 44000 + 1 * 16000, SDS-PAGE Acetobacter pasteurianus
45000
-
1 * 72000 + 1 * 45000, SDS-PAGE Komagataeibacter europaeus
45000
-
1 * 72000 + 1 * 45000, SDS-PAGE Komagataeibacter intermedius
72000
-
1 * 72000 + 1 * 45000, SDS-PAGE Komagataeibacter europaeus
72000
-
1 * 72000 + 1 * 45000, SDS-PAGE Komagataeibacter intermedius
74000
-
1 * 74000 + 1 * 44000 + 1 * 16000, SDS-PAGE Acetobacter pasteurianus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Acetobacter pasteurianus the enzyme activity is correlated with resistance to acetic acid, due to lower enzyme activity in the organism, the growth of Acetobacter pasteurianus on high acetic acid concentrations is limited, overview ?
-
?
additional information Komagataeibacter intermedius the enzyme activity is correlated with resistance to acetic acid, due to lower enzyme activity in the organism, the growth of Gluconacetobacter intermedius on high acetic acid concentrations is limited, overview ?
-
?
additional information Komagataeibacter europaeus high alcohol dehydrogenase activity in the Gluconacetobacter europaeus cells and high acetic acid stability of the purified enzyme represent two of the unique features that enable this species to grow and stay metabolically active at extremely high concentrations of acetic acid ?
-
?
additional information Komagataeibacter intermedius JK3 the enzyme activity is correlated with resistance to acetic acid, due to lower enzyme activity in the organism, the growth of Gluconacetobacter intermedius on high acetic acid concentrations is limited, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Acetobacter pasteurianus Q335W4 AdhA fragment; strains KKP/584 and DSM 3509, gene adhA
-
Komagataeibacter europaeus Q44002 strains VA and DSM 6160, gene adh
-
Komagataeibacter intermedius Q335V9 adh, fragment; gene adh
-
Komagataeibacter intermedius JK3 Q335V9 adh, fragment; gene adh
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from strain JK3, by anion exchange and hydroxylapatite chromatography Komagataeibacter intermedius
native enzyme from strain KKP/584, by anion exchange and hydroxylapatite chromatography Acetobacter pasteurianus
native enzyme from strain V3, by anion exchange and hydroxylapatite chromatography Komagataeibacter europaeus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture the cells show high enzyme activity Komagataeibacter europaeus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
179
-
purified enzyme Komagataeibacter europaeus
192
-
purified enzyme Komagataeibacter intermedius
205
-
purified enzyme Acetobacter pasteurianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + 2,6-dichlorophenol indophenol with phenazine methosulfonate Komagataeibacter europaeus acetaldehyde + reduced 2,6-dichlorophenol indophenol
-
?
ethanol + 2,6-dichlorophenol indophenol with phenazine methosulfonate Komagataeibacter intermedius acetaldehyde + reduced 2,6-dichlorophenol indophenol
-
?
ethanol + 2,6-dichlorophenol indophenol with phenazine methosulfonate Acetobacter pasteurianus acetaldehyde + reduced 2,6-dichlorophenol indophenol
-
?
ethanol + 2,6-dichlorophenol indophenol with phenazine methosulfonate Komagataeibacter intermedius JK3 acetaldehyde + reduced 2,6-dichlorophenol indophenol
-
?
additional information the enzyme activity is correlated with resistance to acetic acid, due to lower enzyme activity in the organism, the growth of Acetobacter pasteurianus on high acetic acid concentrations is limited, overview Acetobacter pasteurianus ?
-
?
additional information the enzyme activity is correlated with resistance to acetic acid, due to lower enzyme activity in the organism, the growth of Gluconacetobacter intermedius on high acetic acid concentrations is limited, overview Komagataeibacter intermedius ?
-
?
additional information high alcohol dehydrogenase activity in the Gluconacetobacter europaeus cells and high acetic acid stability of the purified enzyme represent two of the unique features that enable this species to grow and stay metabolically active at extremely high concentrations of acetic acid Komagataeibacter europaeus ?
-
?
additional information the enzyme activity is correlated with resistance to acetic acid, due to lower enzyme activity in the organism, the growth of Gluconacetobacter intermedius on high acetic acid concentrations is limited, overview Komagataeibacter intermedius JK3 ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 72000 + 1 * 45000, SDS-PAGE Komagataeibacter europaeus
dimer 1 * 72000 + 1 * 45000, SDS-PAGE Komagataeibacter intermedius
trimer 1 * 74000 + 1 * 44000 + 1 * 16000, SDS-PAGE Acetobacter pasteurianus

Synonyms

Synonyms Comment Organism
AdhA
-
Acetobacter pasteurianus
ADHI
-
Komagataeibacter europaeus
PQQ-dependent ADH
-
Komagataeibacter europaeus
PQQ-dependent ADH
-
Komagataeibacter intermedius
PQQ-dependent ADH
-
Acetobacter pasteurianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Komagataeibacter europaeus
7
-
assay at Komagataeibacter intermedius
7
-
assay at Acetobacter pasteurianus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone
-
Acetobacter pasteurianus
pyrroloquinoline quinone dependent on Komagataeibacter europaeus
pyrroloquinoline quinone dependent on Komagataeibacter intermedius