Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Phospholipid | the nature of the phospholipid required to activate the enzyme depends on the nature of the quinone used in the assay system. When 2-methyl-1,4-naphthoquinone is used, a wide variety of phospholipids, including all these isolated from the organism, will activate the enzyme, but when coenzyme Q9 is used the phospholipid specificity of the enzyme is much more restricted, and the most effective activator is the unsaturated phosphatidylethanolamine isolated from the organism | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
ubiquinone 9 | pH 7.0, 20°C | Pseudomonas putida | |
0.45 | - |
(S)-malate | pH 7.0, 20°C | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to the cell-wall membrane | Pseudomonas putida | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
Chester | - |
Pseudomonas putida Chester | - |
Chester | - |
Purification (Comment) | Organism |
---|---|
partial | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + oxidized 2,6-dichlorophenol indophenol | - |
Pseudomonas putida | oxaloacetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
(S)-malate + oxidized 2,6-dichlorophenol indophenol | - |
Pseudomonas putida Chester | oxaloacetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
(S)-malate + ubiquinone-9 | in the presence of both FAD and phospholipid the enzyme catalyzes the reduction of quinone by L-malate at rates equivalent to these obtained with 2,6-dichlorophenol-indophenol as terminal acceptor | Pseudomonas putida | oxaloacetate + ubiquinol-9 | - |
? | |
(S)-malate + ubiquinone-9 | in the presence of both FAD and phospholipid the enzyme catalyzes the reduction of quinone by L-malate at rates equivalent to these obtained with 2,6-dichlorophenol-indophenol as terminal acceptor | Pseudomonas putida Chester | oxaloacetate + ubiquinol-9 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-malate-quinone oxidoreductase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | triple cofactor requirement for FAD, quinone and phospholipid. The formation of reduced forms of FAD is not detected, but in the presence of both FAD and phospholipid the enzyme catalyzes the reduction of quinone by L-malate at rates equivalent to the rate obtained with 2,6-dichlorophenol-indophenol as terminal acceptor. Km-value for FAD is 0.0004 mM | Pseudomonas putida | |
menadione | triple cofactor requirement for FAD, quinone and phospholipid. Maximum rate when phosphatidylethanolamine is added to the enzyme before the quinone | Pseudomonas putida | |
ubiquinone-0 | triple cofactor requirement for FAD, quinone and phospholipid. Maximum activation rate when phosphatidylethanolamine is added to the enzyme before the quinone | Pseudomonas putida | |
ubiquinone-9 | triple cofactor requirement for FAD, quinone and phospholipid. The formation of reduced forms of FAD is not detected, but in the presence of both FAD and phospholipid the enzyme catalyzes the reduction of quinone by L-malate at rates equivalent to the rate obtained with 2,6-dichlorophenol-indophenol as terminal acceptor. The quinone is identified as ubiquinone 9. Km-value for ubiquinone 9 is 0.0024 mM | Pseudomonas putida | |
vitamin K1 | with both vitamin K1 and ubiquinone-9, maximum rates are obtained by exposing the enzyme to phospholipid and quinone simultaneously, but, when phosphatidylethanolamine is added to the enzyme before either of these quinones, the rates are much lower | Pseudomonas putida |