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Literature summary for 1.1.5.4 extracted from
- FAD-dependent malate dehydrogenase, a phospholipid-requiring enzyme from Mycobacterium sp. strain Takeo. Purification and some properties (1978), Biochim. Biophys. Acta, 523, 37-46.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Phospholipid | in absence of either cardiolipin or vitamin K-3 the enzyme shows about 3% of maximal activity | Mycobacterium sp. |  |
| vitamin K3 | in absence of either cardiolipin or vitamin K-3 the enzyme shows about 3% of maximal activity | Mycobacterium sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | 55000 | gel filtration, sucrose density gradient centrifugation | Mycobacterium sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium sp. | - |
- |
- |
| Mycobacterium sp. Takeo | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycobacterium sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 310 | - |
- |
Mycobacterium sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.3 M potassium phosphate buffer, pH 6.6, about 20% loss of activity after 2 weeks | Mycobacterium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + 2,6-dichlorophenol indophenol | - |
Mycobacterium sp. | oxaloacetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
| (S)-malate + 2,6-dichlorophenol indophenol | - |
Mycobacterium sp. Takeo | oxaloacetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAD-dependent malate dehydrogenase | - |
Mycobacterium sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | in absence of FAD no reduction of 2,6-dichlorophenol indophenol is observed | Mycobacterium sp. | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mycobacterium sp. | isoelectric focusing, pH-range 6-8 | - |
6.9 |
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