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Literature summary for 1.1.5.2 extracted from
- Highly conserved Asp-204 and Gly-776 are important for activity of the quinoprotein glucose dehydrogenase of Escherichia coli and for mineral phosphate solubilization (2010), J. Mol. Microbiol. Biotechnol., 18, 109-119.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli AT15 cells | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D204A | no GDH activity | Escherichia coli |
| E217A | no GDH activity | Escherichia coli |
| E217L | no GDH activity | Escherichia coli |
| E217Q | the mutant retains its function similar to that of wild type GDH | Escherichia coli |
| E591K | no GDH activity | Escherichia coli |
| E591L | no GDH activity | Escherichia coli |
| E591Q | no GDH activity | Escherichia coli |
| G776A | the mutant retains its function similar to that of wild type GDH | Escherichia coli |
| G776D | no GDH activity | Escherichia coli |
| G776K | no GDH activity | Escherichia coli |
| G776L | no GDH activity | Escherichia coli |
| L712R | no GDH activity | Escherichia coli |
| L712W | no GDH activity | Escherichia coli |
| R201A | no GDH activity | Escherichia coli |
| R266E | no GDH activity | Escherichia coli |
| R266Q | no GDH activity | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + ubiquinone | Escherichia coli | - |
D-glucono-1,5-lactone + ubiquinol | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + 2,6-dichloroindophenol | - |
Escherichia coli | D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
| D-glucose + ubiquinone | - |
Escherichia coli | D-glucono-1,5-lactone + ubiquinol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDH | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | i.e. 2,7,9,-tricarboxyl-1H-pyrrolo[2,3-f]quinoline-4,5-dione | Escherichia coli | |
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Release 2023.1 (January 2023)
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