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Literature summary for 1.1.5.2 extracted from
- Construction and characterization of heterodimeric soluble quinoprotein glucose dehydrogenase (2004), J. Biochem. Biophys. Methods, 61, 331-338.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of isozyme PQQGDH-B with and without an polyarginine tail in Escherichia coli strain PP2418, and of mutant H168Q | Acinetobacter calcoaceticus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H168Q | site-directed mutagenesis, inactive mutant, a heterodimeric chimeric enzyme consisiting of 1 wild-type subunit and 1 mutant subunit shows decreased activity and a substrate specificity similar to the wild-type enzyme | Acinetobacter calcoaceticus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, cooperativity in the recombinant chimeric mutant enzyme which possesses only 1 active subunit derived from the wild-type enzyme | Acinetobacter calcoaceticus | |
| 2 | 3 | D-glucose | recombinant Arg-tagged enzyme, pH 7.0 | Acinetobacter calcoaceticus |  |
| 2 | 3 | 3-O-methyl-D-glucose | recombinant Arg-tagged enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
| 8 | - |
D-galactose | recombinant Arg-tagged enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
| 9 | - |
D-galactose | recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
| 13 | - |
maltose | recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
| 14 | - |
maltose | recombinant Arg-tagged enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
| 20 | - |
lactose | recombinant Arg-tagged enzyme, and recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
| 22 | - |
D-glucose | recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
| 22 | - |
3-O-methyl-D-glucose | recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
| 34 | - |
allose | recombinant Arg-tagged enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
| 36 | - |
allose | recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | isozyme PQQGDH-B | Acinetobacter calcoaceticus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Acinetobacter calcoaceticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
isozyme PQQGDH-B | - |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| protein dissociation and redimerization of recombinant wild-type and Arg-tagged enzyme, and the mutant H168Q, overview | Acinetobacter calcoaceticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2642 | - |
purified recombinant mutant chimeric isozyme PQQGDH-B | Acinetobacter calcoaceticus |
| 4512 | - |
purified recombinant Arg-tagged isozyme PQQGDH-B | Acinetobacter calcoaceticus |
| 4610 | - |
purified recombinant wild-type isozyme PQQGDH-B | Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-O-methyl-D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | 3-O-methyl-D-glucono-1,5-lactone + ? | - |
? | |
| allose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
| D-galactose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | D-galactono-1,5-lactone + ? | - |
? | |
| D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
| D-glucose + ubiquinone | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| lactose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
| maltose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
| PQQGDH-B | - |
Acinetobacter calcoaceticus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3778 | - |
D-glucose | recombinant Arg-tagged enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
| 4424 | - |
D-glucose | recombinant chimeric mutant enzyme, pH 7.0 | Acinetobacter calcoaceticus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Acinetobacter calcoaceticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyrroloquinoline quinone | i.e. PQQ, dependent on | Acinetobacter calcoaceticus | |
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