Application | Comment | Organism |
---|---|---|
biotechnology | optimization of an expression system using Pichia pastoris for use in industrial level production | Acinetobacter calcoaceticus |
Cloned (Comment) | Organism |
---|---|
expression of isozyme PQQGDH-B, in Pichia pastoris using the alpha-factor signal sequence of Saccharomyces cerevisiae, recombinant enzyme is secreted to the medium, optimization of enzyme production | Acinetobacter calcoaceticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | poor inhibition of isozyme PQQGDH-B by 5 mM EDTA | Acinetobacter calcoaceticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | isozyme PQQGDH-A | Acinetobacter calcoaceticus | 16020 | - |
periplasm | - |
Acinetobacter calcoaceticus | - |
- |
soluble | isozyme PQQGDH-B | Acinetobacter calcoaceticus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, deglycosylated recombinant isozyme PQQGDH-B, SDS-PAGE | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
2 isozymes PQQGDH-A and PQQGDH-B | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | secreted recombinant isozyme PQQGDH-B expressed in Pichia pastoris | Acinetobacter calcoaceticus |
Purification (Comment) | Organism |
---|---|
recombinant isozyme PQQGDH-B from Pichia pastoris culture medium, optimization of down-stream processing | Acinetobacter calcoaceticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5080 | - |
purified recombinant isozyme PQQGDH-B | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + ubiquinone | low activity, recombinant isozyme PQQGDH-B | Acinetobacter calcoaceticus | 2-deoxy-D-glucono-1,5-lactone + ubiquinol | - |
? | |
3-O-methyl-D-glucose + ubiquinone | recombinant isozyme PQQGDH-B | Acinetobacter calcoaceticus | 3-O-methyl-D-glucono-1,5-lactone + ubiquinol | - |
? | |
allose + ubiquinone | recombinant isozyme PQQGDH-B | Acinetobacter calcoaceticus | ? + ubiquinol | - |
? | |
cellobiose + ubiquinone | recombinant isozyme PQQGDH-B | Acinetobacter calcoaceticus | ? + ubiquinol | - |
? | |
D-galactose + ubiquinone | recombinant isozyme PQQGDH-B | Acinetobacter calcoaceticus | D-galactono-1,5-lactone + ubiquinol | - |
? | |
D-glucose + ubiquinone | recombinant isozyme PQQGDH-B | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-mannose + ubiquinone | recombinant isozyme PQQGDH-B | Acinetobacter calcoaceticus | ? + ubiquinol | - |
? | |
D-xylose + ubiquinone | recombinant isozyme PQQGDH-B | Acinetobacter calcoaceticus | D-xylono-1,5-lactone + ubiquinol | - |
? | |
lactose + ubiquinone | recombinant isozyme PQQGDH-B | Acinetobacter calcoaceticus | ? + ubiquinol | - |
? | |
maltose + ubiquinone | recombinant isozyme PQQGDH-B | Acinetobacter calcoaceticus | ? + ubiquinol | - |
? | |
additional information | substrate specificity of the recombinant enzyme produced in Pichia pastoris | Acinetobacter calcoaceticus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, deglycosylated recombinant isozyme PQQGDH-B, SDS-PAGE | Acinetobacter calcoaceticus |
Synonyms | Comment | Organism |
---|---|---|
PQQGDH-B | - |
Acinetobacter calcoaceticus |
pyrroloquinoline quinone glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
isozyme PQQGDH-B, 50% residual activity after 10 min | Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | prosthetic group | Acinetobacter calcoaceticus |