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Literature summary for 1.1.5.2 extracted from

  • Yoshida, H.; Araki, N.; Tomisaka, A.; Sode, K.
    Secretion of water soluble pyrroloquinoline quinone glucose dehydrogenase by recombinant Pichia pastoris (2002), Enzyme Microb. Technol., 30, 312-318.
No PubMed abstract available

Application

Application Comment Organism
biotechnology optimization of an expression system using Pichia pastoris for use in industrial level production Acinetobacter calcoaceticus

Cloned(Commentary)

Cloned (Comment) Organism
expression of isozyme PQQGDH-B, in Pichia pastoris using the alpha-factor signal sequence of Saccharomyces cerevisiae, recombinant enzyme is secreted to the medium, optimization of enzyme production Acinetobacter calcoaceticus

Inhibitors

Inhibitors Comment Organism Structure
additional information poor inhibition of isozyme PQQGDH-B by 5 mM EDTA Acinetobacter calcoaceticus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane isozyme PQQGDH-A Acinetobacter calcoaceticus 16020
-
periplasm
-
Acinetobacter calcoaceticus
-
-
soluble isozyme PQQGDH-B Acinetobacter calcoaceticus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
2 * 50000, deglycosylated recombinant isozyme PQQGDH-B, SDS-PAGE Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
2 isozymes PQQGDH-A and PQQGDH-B
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein secreted recombinant isozyme PQQGDH-B expressed in Pichia pastoris Acinetobacter calcoaceticus

Purification (Commentary)

Purification (Comment) Organism
recombinant isozyme PQQGDH-B from Pichia pastoris culture medium, optimization of down-stream processing Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5080
-
purified recombinant isozyme PQQGDH-B Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + ubiquinone low activity, recombinant isozyme PQQGDH-B Acinetobacter calcoaceticus 2-deoxy-D-glucono-1,5-lactone + ubiquinol
-
?
3-O-methyl-D-glucose + ubiquinone recombinant isozyme PQQGDH-B Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + ubiquinol
-
?
allose + ubiquinone recombinant isozyme PQQGDH-B Acinetobacter calcoaceticus ? + ubiquinol
-
?
cellobiose + ubiquinone recombinant isozyme PQQGDH-B Acinetobacter calcoaceticus ? + ubiquinol
-
?
D-galactose + ubiquinone recombinant isozyme PQQGDH-B Acinetobacter calcoaceticus D-galactono-1,5-lactone + ubiquinol
-
?
D-glucose + ubiquinone recombinant isozyme PQQGDH-B Acinetobacter calcoaceticus D-glucono-1,5-lactone + ubiquinol
-
?
D-mannose + ubiquinone recombinant isozyme PQQGDH-B Acinetobacter calcoaceticus ? + ubiquinol
-
?
D-xylose + ubiquinone recombinant isozyme PQQGDH-B Acinetobacter calcoaceticus D-xylono-1,5-lactone + ubiquinol
-
?
lactose + ubiquinone recombinant isozyme PQQGDH-B Acinetobacter calcoaceticus ? + ubiquinol
-
?
maltose + ubiquinone recombinant isozyme PQQGDH-B Acinetobacter calcoaceticus ? + ubiquinol
-
?
additional information substrate specificity of the recombinant enzyme produced in Pichia pastoris Acinetobacter calcoaceticus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 50000, deglycosylated recombinant isozyme PQQGDH-B, SDS-PAGE Acinetobacter calcoaceticus

Synonyms

Synonyms Comment Organism
PQQGDH-B
-
Acinetobacter calcoaceticus
pyrroloquinoline quinone glucose dehydrogenase
-
Acinetobacter calcoaceticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
isozyme PQQGDH-B, 50% residual activity after 10 min Acinetobacter calcoaceticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone prosthetic group Acinetobacter calcoaceticus