KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.064 | - |
N-methylphenazonium methyl sulfate | pH 8.5, reaction with D-galactose | Acinetobacter calcoaceticus | |
0.118 | - |
L-arabinose | pH 8.5, reaction with D-glucose | Acinetobacter calcoaceticus | |
0.156 | - |
N-methylphenazonium methyl sulfate | pH 8.5, reaction with D-xylose | Acinetobacter calcoaceticus | |
0.178 | - |
N-methylphenazonium methyl sulfate | pH 8.5, reaction with D-glucose | Acinetobacter calcoaceticus | |
0.22 | - |
N-methylphenazonium methyl sulfate | pH 8.5, reaction with D-fucose | Acinetobacter calcoaceticus | |
0.23 | - |
D-glucose | pH 8.5, reaction with 2,6-dichlorophenolindophenol as electron acceptor | Acinetobacter calcoaceticus | |
0.268 | - |
N-ethylphenazonium ethyl sulfate | pH 8.5, reaction with D-xylose | Acinetobacter calcoaceticus | |
0.278 | - |
N-ethylphenazonium ethyl sulfate | pH 8.5, reaction with D-glucose | Acinetobacter calcoaceticus | |
0.291 | - |
N-ethylphenazonium ethyl sulfate | pH 8.5, reaction with D-fucose | Acinetobacter calcoaceticus | |
0.292 | - |
N-ethylphenazonium ethyl sulfate | pH 8.5, reaction with L-arabinose | Acinetobacter calcoaceticus | |
0.362 | - |
N-methylphenazonium methyl sulfate | pH 8.5, reaction with 2-deoxy-D-glucose | Acinetobacter calcoaceticus | |
4 | - |
D-glucose | pH 8.5, reaction with N-methylphenazonium methyl sulfate or N-ethylphenazonium ethal sulfate as electron acceptor | Acinetobacter calcoaceticus | |
5 | - |
D-fucose | pH 8.5, reaction with N-ethylphenazonium ethyl sulfate as electron acceptor | Acinetobacter calcoaceticus | |
5.9 | - |
D-glucose | pH 8.5, reaction with Wurster Blue as electron acceptor | Acinetobacter calcoaceticus | |
7 | - |
D-xylose | pH 8.5, reaction with N-ethylphenazonium ethyl sulfate as electron acceptor | Acinetobacter calcoaceticus | |
12 | - |
D-xylose | pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor | Acinetobacter calcoaceticus | |
12 | - |
D-fucose | pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor | Acinetobacter calcoaceticus | |
18 | - |
L-arabinose | pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor | Acinetobacter calcoaceticus | |
19 | - |
L-arabinose | pH 8.5, reaction with N-ethylphenazonium ethyl sulfate as electron acceptor | Acinetobacter calcoaceticus | |
19 | - |
D-galactose | pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor | Acinetobacter calcoaceticus | |
22 | - |
2-deoxy-D-glucose | pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor | Acinetobacter calcoaceticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ anchors pyrroloquinoline quinone cofactor to the enzyme protein and activates the bound cofactor | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
612 | - |
- |
Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + oxidized N-methylphenazonium methyl sulfate | - |
Acinetobacter calcoaceticus | 2-deoxy-D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate | - |
? | |
D-fucose + N-ethylphenazonium ethyl sulfate | - |
Acinetobacter calcoaceticus | 6-deoxy-D-galactono-1,5-lactone + ? | - |
? | |
D-fucose + oxidized N-methylphenazonium methyl sulfate | - |
Acinetobacter calcoaceticus | 6-deoxy-D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate | - |
? | |
D-galactose + oxidized N-methylphenazonium methyl sulfate | - |
Acinetobacter calcoaceticus | D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate | - |
? | |
D-glucose + 2,6-dichlorophenol-indophenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
D-glucose + N-ethylphenazonium ethyl sulfate | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
D-glucose + oxidized N-methylphenazonium methyl sulfate | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate | - |
? | |
D-xylose + N-ethylphenazonium ethyl sulfate | - |
Acinetobacter calcoaceticus | D-xylono-1,5-lactone + ? | - |
? | |
D-xylose + oxidized N-methylphenazonium methyl sulfate | - |
Acinetobacter calcoaceticus | D-xylono-1,5-lactone + reduced N-methylphenazonium methyl sulfate | - |
? | |
L-arabinose + N-ethylphenazonium ethyl sulfate | - |
Acinetobacter calcoaceticus | L-arabino-1,5-lactone + ? | - |
? | |
L-arabinose + oxidized N-methylphenazonium methyl sulfate | - |
Acinetobacter calcoaceticus | L-arabino-1,5-lactone + reduced N-methylphenazonium methyl sulfate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | reconstitution of the apoenzyme to full activity is achieved with a stoichiometric amount of pyrroloquinoline quinone. Mg2+ anchors pyrroloquinoline quinone cofactor to the enzyme protein and activates the bound cofactor | Acinetobacter calcoaceticus |