Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a gene consisting of two identical subunits linked together by a DNA segment coding linker peptide region and production of a linked-dimeric enzyme, the linked-dimeric enzyme shows higher thermal stability than native dimeric enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
lactose | pH 7.0, 25°C, linked dimeric enzyme | Escherichia coli | |
20 | - |
D-glucose | pH 7.0, 25°C, linked dimeric enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
linked-dimeric enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + ubiquinone | - |
Escherichia coli | ? + ubiquinol | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
20 min, 20% loss of activity of native enzyme, about 50% loss of activity of linked-dimeric enzyme | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | prosthetic group | Escherichia coli |