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Literature summary for 1.1.5.2 extracted from

  • Dokter, P.; Frank, J.; Duine, J.A.
    Purification and characterization of quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus L.M.D. 79.41 (1986), Biochem. J., 239, 163-167.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.5.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.78
-
 pyrroloquinoline quinone
-
 Acinetobacter calcoaceticus
22
-
 D-glucose
-
 Acinetobacter calcoaceticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, SDS-PAGE Acinetobacter calcoaceticus
94000
-
 gel filtration Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
 type II enzyme
-
Acinetobacter calcoaceticus
-
 LMD 79.41
-
Acinetobacter calcoaceticus LMD 79.41
-
 LMD 79.41
-
Escherichia coli
-
 type I enzyme
-
Gluconobacter oxydans
-
 type II enzyme
-
Klebsiella aerogenes
-
 type I enzyme
-
Pseudomonas sp.
-
 type I enzyme
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acinetobacter calcoaceticus

Reaction

Reaction Comment Organism Reaction ID
D-glucose + ubiquinone = D-glucono-1,5-lactone + ubiquinol ping-pong mechanism Acinetobacter calcoaceticus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
640
-
-
 Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + pyrroloquinoline quinone 70% of the activity with D-glucose Acinetobacter calcoaceticus ?
-
 ?
D-galactose + pyrroloquinoline quinone
-
 Escherichia coli D-galactono-1,5-lactone + pyrroloquinoline quinol
-
 ?
D-galactose + pyrroloquinoline quinone 30% of the activity with D-glucose Acinetobacter calcoaceticus D-galactono-1,5-lactone + pyrroloquinoline quinol
-
 ?
D-galactose + pyrroloquinoline quinone 30% of the activity with D-glucose Acinetobacter calcoaceticus LMD 79.41 D-galactono-1,5-lactone + pyrroloquinoline quinol
-
 ?
D-glucose + 2,6-dichlorophenol-indophenol
-
 Escherichia coli D-glucono-1,5-lactone + ?
-
 ?
D-glucose + 2,6-dichlorophenol-indophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
D-glucose + pyrroloquinoline quinone
-
 Escherichia coli D-glucono-1,5-lactone + pyrroloquinoline quinol
-
 ?
D-glucose + pyrroloquinoline quinone
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + pyrroloquinoline quinol
-
 ?
D-glucose + pyrroloquinoline quinone
-
 Acinetobacter calcoaceticus LMD 79.41 D-glucono-1,5-lactone + pyrroloquinoline quinol
-
 ?
D-melibiose + pyrroloquinoline quinone
-
 Escherichia coli ?
-
 ?
D-melibiose + pyrroloquinoline quinone 10% of the activity with D-glucose Acinetobacter calcoaceticus ?
-
 ?
D-xylose + pyrroloquinoline quinone
-
 Escherichia coli D-xylono-1,5-lactone + pyrroloquinoline quinol
-
 ?
D-xylose + pyrroloquinoline quinone 20% of the activity with D-glucose Acinetobacter calcoaceticus D-xylono-1,5-lactone + pyrroloquinoline quinol
-
 ?
D-xylose + pyrroloquinoline quinone 20% of the activity with D-glucose Acinetobacter calcoaceticus LMD 79.41 D-xylono-1,5-lactone + pyrroloquinoline quinol
-
 ?
L-arabinose + pyrroloquinoline quinone
-
 Escherichia coli L-arabino-1,5-lactone + pyrroloquinoline quinol
-
 ?
L-arabinose + pyrroloquinoline quinone 35% of the activity with D-glucose Acinetobacter calcoaceticus L-arabino-1,5-lactone + pyrroloquinoline quinol
-
 ?
L-arabinose + pyrroloquinoline quinone 35% of the activity with D-glucose Acinetobacter calcoaceticus LMD 79.41 L-arabino-1,5-lactone + pyrroloquinoline quinol
-
 ?
L-rhamnose + pyrroloquinoline quinone 7.5% of the activity with D-glucose Acinetobacter calcoaceticus L-rhamnono-1,5-lactone + pyrroloquinoline quinol
-
 ?
lactose + pyrroloquinoline quinone 65% of the activity with D-glucose Acinetobacter calcoaceticus ?
-
 ?
lactose + pyrroloquinoline quinone 65% of the activity with D-glucose Acinetobacter calcoaceticus LMD 79.41 ?
-
 ?
maltose + pyrroloquinoline quinone 90% of the activity with D-glucose Acinetobacter calcoaceticus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Acinetobacter calcoaceticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 reaction with 2,6-dichlorophenol-indophenol Acinetobacter calcoaceticus
9
-
 reaction with pyrroloquinoline quinone Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone prosthetic group Escherichia coli
pyrroloquinoline quinone prosthetic group Klebsiella aerogenes
pyrroloquinoline quinone prosthetic group Pseudomonas sp.
pyrroloquinoline quinone prosthetic group Acinetobacter calcoaceticus
pyrroloquinoline quinone prosthetic group Gluconobacter oxydans
pyrroloquinoline quinone type I enzyme: pyrroloquinoline quinone can be removed by dialysis against EDTA-containg buffers Escherichia coli
pyrroloquinoline quinone type I enzyme: pyrroloquinoline quinone can be removed by dialysis against EDTA-containg buffers Klebsiella aerogenes
pyrroloquinoline quinone type I enzyme: pyrroloquinoline quinone can be removed by dialysis against EDTA-containg buffers Pseudomonas sp.
pyrroloquinoline quinone type II enzyme: pyrroloquinoline quinone can not be removed by dialysis against EDTA-containg buffers Acinetobacter calcoaceticus
pyrroloquinoline quinone type II enzyme: pyrroloquinoline quinone can not be removed by dialysis against EDTA-containg buffers Gluconobacter oxydans